TY - JOUR
T1 - M-RIP, a novel target of JNK signaling and a requirement for human cancer cell invasion
AU - Ono, Ryoko
AU - Matsuoka, Junji
AU - Yamatsuji, Tomoki
AU - Naomoto, Yoshio
AU - Tanaka, Noriaki
AU - Matsui, Hideki
AU - Matsushita, Masayuki
PY - 2008/8
Y1 - 2008/8
N2 - Cell motility is involved in physiological and pathological processes such as the invasion and migration of cells. c-Jun N-terminal kinase (JNK) cascades are involved in the invasion and metastasis of cancer cells. However, little is known about the downstream signaling of JNK. In the present study, we used small interfering RNA (siRNA) directed against JNK1 to reduce its expression. We used microarray techniques to compare the gene expression profiles of epidermal growth factor (EGF)-stimulated HeLa cells with and without JNK1 siRNA treatment. We identified a JNK1 target gene, myosin phosphatase-Rho interacting protein (M-RIP). RNA interference-mediated inhibition of JNK1 strongly inhibited M-RIP mRNA expression induced by EGF, as well as the invasion of HeLa cells. In addition, M-RIP siRNA-treated cells showed significantly reduced invasive activity. Thus, a functional analysis of JNK1 and M-RIP with RNA interference reveals a critical role for this cascade in the invasive behavior of cancer cells.
AB - Cell motility is involved in physiological and pathological processes such as the invasion and migration of cells. c-Jun N-terminal kinase (JNK) cascades are involved in the invasion and metastasis of cancer cells. However, little is known about the downstream signaling of JNK. In the present study, we used small interfering RNA (siRNA) directed against JNK1 to reduce its expression. We used microarray techniques to compare the gene expression profiles of epidermal growth factor (EGF)-stimulated HeLa cells with and without JNK1 siRNA treatment. We identified a JNK1 target gene, myosin phosphatase-Rho interacting protein (M-RIP). RNA interference-mediated inhibition of JNK1 strongly inhibited M-RIP mRNA expression induced by EGF, as well as the invasion of HeLa cells. In addition, M-RIP siRNA-treated cells showed significantly reduced invasive activity. Thus, a functional analysis of JNK1 and M-RIP with RNA interference reveals a critical role for this cascade in the invasive behavior of cancer cells.
KW - Eukaryotic initiation factor
KW - Focal adhesion kinase
KW - Jun
KW - Myosin phosphatase-Rho interacting protein
KW - Small interfering RNA
UR - http://www.scopus.com/inward/record.url?scp=48749083662&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=48749083662&partnerID=8YFLogxK
U2 - 10.3892/ijmm_00000009
DO - 10.3892/ijmm_00000009
M3 - Article
C2 - 18636174
AN - SCOPUS:48749083662
SN - 1107-3756
VL - 22
SP - 199
EP - 203
JO - International journal of molecular medicine
JF - International journal of molecular medicine
IS - 2
ER -
