Mechanical strain regulates endothelial cell patterning in vitro

Takuya Matsumoto, Yu Ching Yung, Claudia Fischbach, Hyun Joon Kong, Ryusuke Nakaoka, David J. Mooney

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)


Blood vessels of the vertebrate circulatory system typically exhibit tissue-specific patterning. However, the cues that guide the development of these patterns remain unclear. We investigated the effect of cyclic uniaxial strain on vascular endothelial cell dynamics and sprout formation in vitro in two-dimensional (2D) and three-dimensional (3D) culture systems under the infuence of growth factors. Cells preferentially aligned and moved in the direction perpendicular to the major strain axis in monolayer culture, and mechanical strain also regulated the spatial location of cell proliferation in 2D cell culture. Cells in 3D cell culture could be induced to form sprouts by exposure to appropriate growth factor combinations (vascular endothelial growth factor and hepatocyte growth factor), and the strain direction regulated the directionality of this process. Moreover, cyclic uniaxial strain inhibited branching of the structures formed by endothelial cells and increased their thickness. Taken together, these data support the importance of external mechanical stimulation in the regulation of endothelial cell migration, proliferation, and differentiation into primitive vessels.

Original languageEnglish
Pages (from-to)207-217
Number of pages11
JournalTissue Engineering
Issue number1
Publication statusPublished - Jan 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Cell Biology


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