Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium

Ezat Asgarani; Hirofumi Funamizu; Takeshi Saito; Hiroaki Terato; Yoshihiko Ohyama; Osamu Yamamoto; Ide Hiroshi

doi:10.1016/S0944-5013(99)80013-5

Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium

Ezat Asgarani, Hirofumi Funamizu, Takeshi Saito, Hiroaki Terato, Yoshihiko Ohyama, Osamu Yamamoto, Ide Hiroshi

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Halobacterium salinarium exhibits resistance to ultraviolet (UV) and ionizing radiation. This organism contains carotenoids and also accumulates highly concentrated KCl in the cell. In the present study, DNA lesions generated by UV and ionizing radiation were measured in vitro in the presence and absence of bacterioruberin, the major carotenoid of H. salinarium, and KCl to elucidate their influences on DNA damage production. When plasmid DNA (pDEL19) was UV-irradiated, formation of cyclobutane pyrimidine dimers (CPD) was slightly suppressed by 0.1 mM bacterioruberin. The same concentration of bacterioruberin suppressed the formation of DNA single strand breaks (SSB) by ionizing radiation more efficiently. The formation of CPD by UV and SSB by ionizing radiation was also repressed by 2M KCl but protection against ionizing radiation was extremely efficient.

Original language	English
Pages (from-to)	185-190
Number of pages	6
Journal	Microbiological Research
Volume	154
Issue number	2
DOIs	https://doi.org/10.1016/S0944-5013(99)80013-5
Publication status	Published - Sept 1999
Externally published	Yes

Keywords

Carotenoid
DNA damage
Halophilic bacteria
Intracellular salt
Resistance

ASJC Scopus subject areas

Microbiology

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10.1016/S0944-5013(99)80013-5

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title = "Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium",

abstract = "Halobacterium salinarium exhibits resistance to ultraviolet (UV) and ionizing radiation. This organism contains carotenoids and also accumulates highly concentrated KCl in the cell. In the present study, DNA lesions generated by UV and ionizing radiation were measured in vitro in the presence and absence of bacterioruberin, the major carotenoid of H. salinarium, and KCl to elucidate their influences on DNA damage production. When plasmid DNA (pDEL19) was UV-irradiated, formation of cyclobutane pyrimidine dimers (CPD) was slightly suppressed by 0.1 mM bacterioruberin. The same concentration of bacterioruberin suppressed the formation of DNA single strand breaks (SSB) by ionizing radiation more efficiently. The formation of CPD by UV and SSB by ionizing radiation was also repressed by 2M KCl but protection against ionizing radiation was extremely efficient.",

keywords = "Carotenoid, DNA damage, Halophilic bacteria, Intracellular salt, Resistance",

author = "Ezat Asgarani and Hirofumi Funamizu and Takeshi Saito and Hiroaki Terato and Yoshihiko Ohyama and Osamu Yamamoto and Ide Hiroshi",

year = "1999",

month = sep,

doi = "10.1016/S0944-5013(99)80013-5",

language = "English",

volume = "154",

pages = "185--190",

journal = "Microbiological Research",

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publisher = "Urban und Fischer Verlag Jena",

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T1 - Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium

AU - Asgarani, Ezat

AU - Funamizu, Hirofumi

AU - Saito, Takeshi

AU - Terato, Hiroaki

AU - Ohyama, Yoshihiko

AU - Yamamoto, Osamu

AU - Hiroshi, Ide

PY - 1999/9

Y1 - 1999/9

N2 - Halobacterium salinarium exhibits resistance to ultraviolet (UV) and ionizing radiation. This organism contains carotenoids and also accumulates highly concentrated KCl in the cell. In the present study, DNA lesions generated by UV and ionizing radiation were measured in vitro in the presence and absence of bacterioruberin, the major carotenoid of H. salinarium, and KCl to elucidate their influences on DNA damage production. When plasmid DNA (pDEL19) was UV-irradiated, formation of cyclobutane pyrimidine dimers (CPD) was slightly suppressed by 0.1 mM bacterioruberin. The same concentration of bacterioruberin suppressed the formation of DNA single strand breaks (SSB) by ionizing radiation more efficiently. The formation of CPD by UV and SSB by ionizing radiation was also repressed by 2M KCl but protection against ionizing radiation was extremely efficient.

AB - Halobacterium salinarium exhibits resistance to ultraviolet (UV) and ionizing radiation. This organism contains carotenoids and also accumulates highly concentrated KCl in the cell. In the present study, DNA lesions generated by UV and ionizing radiation were measured in vitro in the presence and absence of bacterioruberin, the major carotenoid of H. salinarium, and KCl to elucidate their influences on DNA damage production. When plasmid DNA (pDEL19) was UV-irradiated, formation of cyclobutane pyrimidine dimers (CPD) was slightly suppressed by 0.1 mM bacterioruberin. The same concentration of bacterioruberin suppressed the formation of DNA single strand breaks (SSB) by ionizing radiation more efficiently. The formation of CPD by UV and SSB by ionizing radiation was also repressed by 2M KCl but protection against ionizing radiation was extremely efficient.

KW - Carotenoid

KW - DNA damage

KW - Halophilic bacteria

KW - Intracellular salt

KW - Resistance

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U2 - 10.1016/S0944-5013(99)80013-5

DO - 10.1016/S0944-5013(99)80013-5

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VL - 154

SP - 185

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JO - Microbiological Research

JF - Microbiological Research

IS - 2

ER -

Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium

Abstract

Keywords

ASJC Scopus subject areas

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