Mechanosensitive ion channel MscL controls ionic fluxes during cold and heat stress in Synechocystis

Dmitry Bachin; Lyudmila V. Nazarenko; Kirill S. Mironov; Tatiana Pisareva; Suleyman I. Allakhverdiev; Dmitry A. Los

doi:10.1093/femsle/fnv090

Mechanosensitive ion channel MscL controls ionic fluxes during cold and heat stress in Synechocystis

Dmitry Bachin, Lyudmila V. Nazarenko, Kirill S. Mironov, Tatiana Pisareva, Suleyman I. Allakhverdiev, Dmitry A. Los

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Calcium plays an essential role in a variety of stress responses of eukaryotic cells; however, its function in prokaryotes is obscure. Bacterial ion channels that transport Ca²⁺ are barely known. We investigated temperature-induced changes in intracellular concentration of Ca²⁺, Na⁺ and K⁺ in the cyanobacterium Synechocystis sp. strain PCC 6803 and its mutant that is defective in mechanosensitive ion channel MscL. Concentration of cations rapidly and transiently increased in wild-type cells in response to cold and heat treatments. These changes in ionic concentrations correlated with the changes in cytoplasmic volume that transiently decreased in response to temperature treatments. However, no increase in ionic concentrations was observed in the MscL-mutant cells. It implies that MscL functions as a non-specific ion channel, and it participates in regulation of cell volume under temperature-stress conditions.

Original language	English
Article number	fnv090
Journal	FEMS Microbiology Letters
Volume	362
Issue number	12
DOIs	https://doi.org/10.1093/femsle/fnv090
Publication status	Published - Jun 1 2015

Keywords

Calcium
Cold stress
Cyanobacteria
Heat stress
Ion channels
MscL

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1093/femsle/fnv090

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title = "Mechanosensitive ion channel MscL controls ionic fluxes during cold and heat stress in Synechocystis",

abstract = "Calcium plays an essential role in a variety of stress responses of eukaryotic cells; however, its function in prokaryotes is obscure. Bacterial ion channels that transport Ca2+ are barely known. We investigated temperature-induced changes in intracellular concentration of Ca2+, Na+ and K+ in the cyanobacterium Synechocystis sp. strain PCC 6803 and its mutant that is defective in mechanosensitive ion channel MscL. Concentration of cations rapidly and transiently increased in wild-type cells in response to cold and heat treatments. These changes in ionic concentrations correlated with the changes in cytoplasmic volume that transiently decreased in response to temperature treatments. However, no increase in ionic concentrations was observed in the MscL-mutant cells. It implies that MscL functions as a non-specific ion channel, and it participates in regulation of cell volume under temperature-stress conditions.",

keywords = "Calcium, Cold stress, Cyanobacteria, Heat stress, Ion channels, MscL",

author = "Dmitry Bachin and Nazarenko, {Lyudmila V.} and Mironov, {Kirill S.} and Tatiana Pisareva and Allakhverdiev, {Suleyman I.} and Los, {Dmitry A.}",

year = "2015",

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doi = "10.1093/femsle/fnv090",

language = "English",

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T1 - Mechanosensitive ion channel MscL controls ionic fluxes during cold and heat stress in Synechocystis

AU - Bachin, Dmitry

AU - Nazarenko, Lyudmila V.

AU - Mironov, Kirill S.

AU - Pisareva, Tatiana

AU - Allakhverdiev, Suleyman I.

AU - Los, Dmitry A.

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Calcium plays an essential role in a variety of stress responses of eukaryotic cells; however, its function in prokaryotes is obscure. Bacterial ion channels that transport Ca2+ are barely known. We investigated temperature-induced changes in intracellular concentration of Ca2+, Na+ and K+ in the cyanobacterium Synechocystis sp. strain PCC 6803 and its mutant that is defective in mechanosensitive ion channel MscL. Concentration of cations rapidly and transiently increased in wild-type cells in response to cold and heat treatments. These changes in ionic concentrations correlated with the changes in cytoplasmic volume that transiently decreased in response to temperature treatments. However, no increase in ionic concentrations was observed in the MscL-mutant cells. It implies that MscL functions as a non-specific ion channel, and it participates in regulation of cell volume under temperature-stress conditions.

AB - Calcium plays an essential role in a variety of stress responses of eukaryotic cells; however, its function in prokaryotes is obscure. Bacterial ion channels that transport Ca2+ are barely known. We investigated temperature-induced changes in intracellular concentration of Ca2+, Na+ and K+ in the cyanobacterium Synechocystis sp. strain PCC 6803 and its mutant that is defective in mechanosensitive ion channel MscL. Concentration of cations rapidly and transiently increased in wild-type cells in response to cold and heat treatments. These changes in ionic concentrations correlated with the changes in cytoplasmic volume that transiently decreased in response to temperature treatments. However, no increase in ionic concentrations was observed in the MscL-mutant cells. It implies that MscL functions as a non-specific ion channel, and it participates in regulation of cell volume under temperature-stress conditions.

KW - Calcium

KW - Cold stress

KW - Cyanobacteria

KW - Heat stress

KW - Ion channels

KW - MscL

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ER -

Mechanosensitive ion channel MscL controls ionic fluxes during cold and heat stress in Synechocystis

Abstract

Keywords

ASJC Scopus subject areas

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