TY - JOUR
T1 - Meiotic inheritance of a fungal supernumerary chromosome and its effect on sexual fertility in Nectria haematococca
AU - Garmaroodi, Hamid S.
AU - Taga, Masatoki
N1 - Funding Information:
We would like to thank Profs. H.D. VanEtten for providing the strains and J.P.M. Camacho for fruitful discussions. This work was supported in part by Japan Student Services Organization (JASSO) as a fellowship to HSG.
Publisher Copyright:
© 2015 The British Mycological Society.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - PDA1-conditionally dispensable chromosome (CDC) of Nectria haematococca MP VI has long served as a model of supernumerary chromosomes in plant pathogenic fungi because of pathogenicity-related genes located on it. In our previous study, we showed the dosage effects of PDA1-CDC on pathogenicity and homoserine utilization by exploiting tagged PDA1-CDC with a marker gene. CDC content of mating partners and progenies analyzed by PCR, PFGE combined with Southern analysis and chromosome painting via FISH. In this study, we analyzed mode of meiotic inheritance of PDA1-CDC in several mating patterns with regard to CDC content and found a correlation between CDC content of parental strains with fertility of crosses. The results showed non-Mendelian inheritance of this chromosome followed by duplication or loss of the CDC in haploid genome through meiosis that probably were due to premature centromere division, not by nondisjunction as reported for the supernumerary chromosomes in other species. Correlation of CDC with fertility is the first time to be examined in fungi in this study.
AB - PDA1-conditionally dispensable chromosome (CDC) of Nectria haematococca MP VI has long served as a model of supernumerary chromosomes in plant pathogenic fungi because of pathogenicity-related genes located on it. In our previous study, we showed the dosage effects of PDA1-CDC on pathogenicity and homoserine utilization by exploiting tagged PDA1-CDC with a marker gene. CDC content of mating partners and progenies analyzed by PCR, PFGE combined with Southern analysis and chromosome painting via FISH. In this study, we analyzed mode of meiotic inheritance of PDA1-CDC in several mating patterns with regard to CDC content and found a correlation between CDC content of parental strains with fertility of crosses. The results showed non-Mendelian inheritance of this chromosome followed by duplication or loss of the CDC in haploid genome through meiosis that probably were due to premature centromere division, not by nondisjunction as reported for the supernumerary chromosomes in other species. Correlation of CDC with fertility is the first time to be examined in fungi in this study.
KW - Conditionally dispensable chromosome (CDC)
KW - FISH
KW - Nondisjunction
UR - http://www.scopus.com/inward/record.url?scp=84942036382&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84942036382&partnerID=8YFLogxK
U2 - 10.1016/j.funbio.2015.07.004
DO - 10.1016/j.funbio.2015.07.004
M3 - Article
C2 - 26399187
AN - SCOPUS:84942036382
SN - 1878-6146
VL - 119
SP - 929
EP - 939
JO - Mycological Research
JF - Mycological Research
IS - 10
M1 - 603
ER -
