Microheterogeneity of regional myocardial blood flows in low-perfused rat hearts evaluated by double-tracer digital radiography

Takeshi Matsumoto, Takahisa Asano, Mami Takemoto, Hiroyuki Tachibana, Yasuo Ogasawara, Fumihiko Kajiya

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2 Citations (Scopus)


Using 3H- and 125I-labeled desmethylimipramine (DMI) for regional flow tracers, we established a two-time measurement method for the spatial pattern of myocardial perfusion in cross-circulated rat hearts. Myocardial extractions and retentions of these tracers were confirmed to be satisfactory; however, the latter were less than 90% after 3 min at a perfusion rate of 2.9 ml/min/g, limiting the present application to a short-time perfusion measurement. Distributions of myocardial depositions were separated by subtraction digital radiography with 400-μm pixel resolution. Its feasibility was examined by regression analysis between local deposition densities of 3H- and 125I-DMI injected simultaneously. The slope, y-intercept, and correlation coefficient (r) of the regression line were 0.98±0.04, 0.02±0.04, and 0.95±0.03, respectively, indicating the validity of the present image subtraction technique. The spatial pattern of myocardial perfusion in response to flow reduction was evaluated by the injections of 3H- and 125I-DMI, respectively, before and after a nearly 70% flow reduction. A significant correlation between normalized density distributions of these tracers was found in both subepicardium (r=0.77±0.12) and subendocardium (r=0.73±0.20), indicating the stable pattern of myocardial perfusion. However, the coefficient of variation of tracer densities showed a decrease of subendocardial flow heterogeneity from 35±15% to 31±16%. Thus, flow differences between originally high- and low-flow regions in subendocardium were reduced on a relative basis during low perfusion.

Original languageEnglish
Pages (from-to)910-917
Number of pages8
JournalApplied Radiation and Isotopes
Issue number8
Publication statusPublished - Aug 2007
Externally publishedYes


  • Low perfusion
  • Microheterogeneity of flows
  • Molecular flow tracer
  • Myocardial retention
  • Quantitative digital radiography

ASJC Scopus subject areas

  • Radiation


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