MicroRNA-351 regulates two-types of cell death, necrosis and apoptosis, induced by 5-fluoro-2'-deoxyuridine

Akira Sato; Takuya Omi; Akihiro Yamamoto; Akito Satake; Akiko Hiramoto; Mitsuko Masutani; Sei Ichi Tanuma; Yusuke Wataya; Hye Sook Kim

doi:10.1371/journal.pone.0153130

MicroRNA-351 regulates two-types of cell death, necrosis and apoptosis, induced by 5-fluoro-2'-deoxyuridine

Akira Sato, Takuya Omi, Akihiro Yamamoto, Akito Satake, Akiko Hiramoto, Mitsuko Masutani, Sei Ichi Tanuma, Yusuke Wataya, Hye Sook Kim

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Cell-death can be necrosis and apoptosis. We are investigating the mechanisms regulating the cell death that occurs on treatment of mouse cancer cell-line FM3A with antitumor 5- fluoro-2'-deoxyuridine (FUdR): necrosis occurs for the original clone F28-7, and apoptosis for its variant F28-7-A. Here we report that a microRNA (miR-351) regulates the cell death pattern. The miR-351 is expressed strongly in F28-7-A but only weakly in F28-7. Induction of a higher expression of miR-351 in F28-7 by transfecting an miRNA mimic into F28-7 resulted in a change of the death mode; necrosis to apoptosis. Furthermore, transfection of an miR-351 inhibitor into F28-7-A resulted in the morphology change, apoptosis to necrosis, in this death-by-FUdR. Possible mechanism involving lamin B1 in this miR-351's regulatory action is discussed.

Original language	English
Article number	e0153130
Journal	PloS one
Volume	11
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0153130
Publication status	Published - Apr 2016

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "MicroRNA-351 regulates two-types of cell death, necrosis and apoptosis, induced by 5-fluoro-2'-deoxyuridine",

abstract = "Cell-death can be necrosis and apoptosis. We are investigating the mechanisms regulating the cell death that occurs on treatment of mouse cancer cell-line FM3A with antitumor 5- fluoro-2'-deoxyuridine (FUdR): necrosis occurs for the original clone F28-7, and apoptosis for its variant F28-7-A. Here we report that a microRNA (miR-351) regulates the cell death pattern. The miR-351 is expressed strongly in F28-7-A but only weakly in F28-7. Induction of a higher expression of miR-351 in F28-7 by transfecting an miRNA mimic into F28-7 resulted in a change of the death mode; necrosis to apoptosis. Furthermore, transfection of an miR-351 inhibitor into F28-7-A resulted in the morphology change, apoptosis to necrosis, in this death-by-FUdR. Possible mechanism involving lamin B1 in this miR-351's regulatory action is discussed.",

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AU - Sato, Akira

AU - Omi, Takuya

AU - Yamamoto, Akihiro

AU - Satake, Akito

AU - Hiramoto, Akiko

AU - Masutani, Mitsuko

AU - Tanuma, Sei Ichi

AU - Wataya, Yusuke

AU - Kim, Hye Sook

N1 - Publisher Copyright: © 2016 Sato et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2016/4

Y1 - 2016/4

N2 - Cell-death can be necrosis and apoptosis. We are investigating the mechanisms regulating the cell death that occurs on treatment of mouse cancer cell-line FM3A with antitumor 5- fluoro-2'-deoxyuridine (FUdR): necrosis occurs for the original clone F28-7, and apoptosis for its variant F28-7-A. Here we report that a microRNA (miR-351) regulates the cell death pattern. The miR-351 is expressed strongly in F28-7-A but only weakly in F28-7. Induction of a higher expression of miR-351 in F28-7 by transfecting an miRNA mimic into F28-7 resulted in a change of the death mode; necrosis to apoptosis. Furthermore, transfection of an miR-351 inhibitor into F28-7-A resulted in the morphology change, apoptosis to necrosis, in this death-by-FUdR. Possible mechanism involving lamin B1 in this miR-351's regulatory action is discussed.

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MicroRNA-351 regulates two-types of cell death, necrosis and apoptosis, induced by 5-fluoro-2'-deoxyuridine

Abstract

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