Milk supplements in a glycerol free trehalose freezing extender enhanced cryosurvival of boar spermatozoa

Rukmali Athurupana, Hiroaki Funahashi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Objective: To evaluate the effect of skim milk and/or coconut milk in a glycerol-free trehalose extender to improve cryosurvival of boar spermatozoa. Methods: Sperm samples were diluted in an egg-yolk-based freezing extender containing 100 mM trehalose and 0.25% Equex STM supplemented with coconut milk or/and skim milk at 2% or 5% (w/v). Spermatozoa were cryopreserved in 0.5 mL straws and thawed by a rapid transient method (at 70 °C for 8 s) followed with a stabilizing procedure at 39 °C. Thawed samples were analyzed for motility, viability, high mitochondrial membrane potential (HMMP), and acrosome damage. Results: Even on the presence of egg yolk, motility, HMMP and viability were significantly higher in extender supplemented with 2% skim milk than controls without skim milk (. P < 0.05). Post-thaw viability significantly improved with the addition of 2% skim milk plus 2% coconut milk as well (. P < 0.05). Acrosome damage was considerably lower when the extender was supplemented with 2% coconut milk (. P < 0.05), whereas the benefit was masked in the presence of 2% skim milk. Conclusion: 2% skim milk can be used as supplements for a glycerol-free trehalose and egg yolk-based extender to improve post-thaw survival of boar spermatozoa, whereas 2% coconut milk has an effect to protect boar spermatozoa from acrosome damage.

Original languageEnglish
Pages (from-to)58-62
Number of pages5
JournalAsian Pacific Journal of Reproduction
Issue number1
Publication statusPublished - Mar 1 2016


  • Cryopreservation
  • Pigs
  • Powdered coconut milk
  • Skim milk
  • Sperm
  • Trehalose

ASJC Scopus subject areas

  • Reproductive Medicine
  • Animal Science and Zoology
  • veterinary(all)
  • Plant Science
  • Obstetrics and Gynaecology


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