TY - JOUR
T1 - Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family
AU - Chen, Zonghui
AU - Fujii, Yumi
AU - Yamaji, Naoki
AU - Masuda, Sakine
AU - Takemoto, Yuma
AU - Kamiya, Takehiro
AU - Yusuyin, Yusufujiang
AU - Iwasaki, Kozo
AU - Kato, Shin Ichiro
AU - Maeshima, Masayoshi
AU - Ma, Jian Feng
AU - Ueno, Daisei
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan (no. 23119515 to DU, no. 22119002 to JFM), and by The Kurata Memorial Hitachi Science and Technology Foundation (No. 1044 to DU). We thank Editage for editorial assistance.
PY - 2013/11
Y1 - 2013/11
N2 - Manganese (Mn) is an essential micronutrient for plants, but is toxic when present in excess. The rice plant (Oryza sativa L.) accumulates high concentrations of Mn in the aerial parts; however, the molecular basis for Mn tolerance is poorly understood. In the present study, genes encoding Mn tolerance were screened for by expressing cDNAs of genes from rice shoots in Saccharomyces cerevisiae. A gene encoding a cation diffusion facilitator (CDF) family member, OsMTP8.1, was isolated, and its expression was found to enhance Mn accumulation and tolerance in S. cer-evisiae. In plants, OsMTP8.1 and its transcript were mainly detected in shoots. High or low supply of Mn moderately induced an increase or decrease in the accumulation of OsMTP8.1, respectively. OsMTP8.1 was detected in all cells of leaf blades through immunohistochemistry. OsMTP8.1 fused to green fluorescent protein was localized to the tonoplast. Disruption of OsMTP8.1 resulted in decreased chlorophyll levels, growth inhibition in the presence of high concentrations of Mn, and decreased accumulation of Mn in shoots and roots. However, there was no difference in the accumulation of other metals, including Zn, Cu, Fe, Mg, Ca, and K. These results suggest that OsMTP8.1 is an Mn-specific transporter that sequesters Mn into vacuoles in rice and is required for Mn tolerance in shoots.
AB - Manganese (Mn) is an essential micronutrient for plants, but is toxic when present in excess. The rice plant (Oryza sativa L.) accumulates high concentrations of Mn in the aerial parts; however, the molecular basis for Mn tolerance is poorly understood. In the present study, genes encoding Mn tolerance were screened for by expressing cDNAs of genes from rice shoots in Saccharomyces cerevisiae. A gene encoding a cation diffusion facilitator (CDF) family member, OsMTP8.1, was isolated, and its expression was found to enhance Mn accumulation and tolerance in S. cer-evisiae. In plants, OsMTP8.1 and its transcript were mainly detected in shoots. High or low supply of Mn moderately induced an increase or decrease in the accumulation of OsMTP8.1, respectively. OsMTP8.1 was detected in all cells of leaf blades through immunohistochemistry. OsMTP8.1 fused to green fluorescent protein was localized to the tonoplast. Disruption of OsMTP8.1 resulted in decreased chlorophyll levels, growth inhibition in the presence of high concentrations of Mn, and decreased accumulation of Mn in shoots and roots. However, there was no difference in the accumulation of other metals, including Zn, Cu, Fe, Mg, Ca, and K. These results suggest that OsMTP8.1 is an Mn-specific transporter that sequesters Mn into vacuoles in rice and is required for Mn tolerance in shoots.
KW - CDF family
KW - Manganese tolerance
KW - OsMTP8.1
KW - Rice
KW - Transporter
KW - Vacuole
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U2 - 10.1093/jxb/ert243
DO - 10.1093/jxb/ert243
M3 - Article
C2 - 23963678
AN - SCOPUS:84887909365
SN - 0022-0957
VL - 64
SP - 4375
EP - 4387
JO - Journal of experimental botany
JF - Journal of experimental botany
IS - 14
ER -