TY - JOUR
T1 - Model of ischemic heart disease and video-based comparison of cardiomyocyte contraction using hipsc-derived cardiomyocytes
AU - Liu, Yun
AU - Liang, Yin
AU - Wang, Mengxue
AU - Wang, Chen
AU - Wei, Heng
AU - Naruse, Keiji
AU - Takahashi, Ken
N1 - Funding Information:
This study was supported by JSPS KAKENHI, Fund for the Promotion of Joint International Research (Fostering Joint International Research), 17KK0168. The authors gratefully acknowledge Central Research Laboratory, Okayama University Medical School for the assistance of FACS.
Publisher Copyright:
© 2020 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
PY - 2020/5
Y1 - 2020/5
N2 - Ischemic heart disease is a significant cause of death worldwide. It has therefore been the subject of a tremendous amount of research, often with small-animal models such as rodents. However, the physiology of the human heart differs significantly from that of the rodent heart, underscoring the need for clinically relevant models to study heart disease. Here, we present a protocol to model ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells (hiPS-CMs) and to quantify the damage and functional impairment of the ischemic cardiomyocytes. Exposure to 2% oxygen without glucose and serum increases the percentage of injured cells, which is indicated by staining of the nucleus with propidium iodide, and decreases cellular viability. These conditions also decrease the contractility of hiPS-CMs as confirmed by displacement vector field analysis of microscopic video images. This protocol may furthermore provide a convenient method for personalized drug screening by facilitating the use of hiPS cells from individual patients. Therefore, this model of ischemic heart disease, based on iPS-CMs of human origin, can provide a useful platform for drug screening and further research on ischemic heart disease.
AB - Ischemic heart disease is a significant cause of death worldwide. It has therefore been the subject of a tremendous amount of research, often with small-animal models such as rodents. However, the physiology of the human heart differs significantly from that of the rodent heart, underscoring the need for clinically relevant models to study heart disease. Here, we present a protocol to model ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells (hiPS-CMs) and to quantify the damage and functional impairment of the ischemic cardiomyocytes. Exposure to 2% oxygen without glucose and serum increases the percentage of injured cells, which is indicated by staining of the nucleus with propidium iodide, and decreases cellular viability. These conditions also decrease the contractility of hiPS-CMs as confirmed by displacement vector field analysis of microscopic video images. This protocol may furthermore provide a convenient method for personalized drug screening by facilitating the use of hiPS cells from individual patients. Therefore, this model of ischemic heart disease, based on iPS-CMs of human origin, can provide a useful platform for drug screening and further research on ischemic heart disease.
KW - Cardiomyocytes
KW - Cellular differentiation
KW - Human induced pluripotent stem cells
KW - Hypoxia
KW - Ischemic heart disease
KW - Issue 159
KW - Medicine
KW - Myocardial infarction
UR - http://www.scopus.com/inward/record.url?scp=85085298156&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85085298156&partnerID=8YFLogxK
U2 - 10.3791/61104
DO - 10.3791/61104
M3 - Article
C2 - 32449739
AN - SCOPUS:85085298156
SN - 1940-087X
VL - 2020
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 159
M1 - e61104
ER -
