TY - JOUR
T1 - Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana
AU - Higashi, Kuniaki
AU - Ishiga, Yasuhiro
AU - Inagaki, Yoshishige
AU - Toyoda, Kazuhiro
AU - Shiraishi, Tomonori
AU - Ichinose, Yuki
N1 - Funding Information:
Acknowledgments We thank Dr. A. Collmer (Cornell University, USA), Dr. J. L. Dangl (North Carolina University, USA), and Dr. S. Tsuyumu (Shizuoka University, Japan) for providing Pto wild-type and its ΔhrcQ-U mutant, Pto::avrRpt2, and Ecc EC1, respectively. We are also grateful to the Leaf Tobacco Research Laboratory, Japan Tobacco Inc., the Plant Cell Bank of The Institute of Physical and Chemical Research (RIKEN), and the Salk Institute Genomic Analysis Laboratory for providing Pta and T-87 A. thaliana suspension-cultured cells and seeds for Xs2- and WRKY41-mutants, respectively. This work was partly supported by Grants-in-Aid for ScientiWc Research (S) (No. 15108001) and (B) (No. 18380035) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, Program for Promotion of Basic Research Activities for Innovative Bioscience (PRO-BRAIN) and the Okayama University COE program “Establishment of Plant Health Science”.
PY - 2008/3
Y1 - 2008/3
N2 - Flagellin, a component of the flagellar filament of Pseudomonas syringae pv. tabaci 6605 (Pta), induces hypersensitive reaction in its non-host Arabidopsis thaliana. We identified the WRKY41 gene, which belongs to a multigene family encoding WRKY plant-specific transcription factors, as one of the flagellin-inducible genes in A. thaliana. Expression of WRKY41 is induced by inoculation with the incompatible pathogen P. syringae pv. tomato DC3000 (Pto) possessing AvrRpt2 and the non-host pathogens Pta within 6-h after inoculation, but not by inoculation with the compatible Pto. Expression of WRKY41 was also induced by inoculation of A. thaliana with an hrp-type three secretion system (T3SS)-defective mutant of Pto, indicating that effectors produced by T3SS in the Pto wild-type suppress the activation of WRKY41. Arabidopsis overexpressing WRKY41 showed enhanced resistance to the Pto wild-type but increased susceptibility to Erwinia carotovora EC1. WRKY41-overexpressing Arabidopsis constitutively expresses the PR5 gene, but suppresses the methyl jasmonate-induced PDF1.2 gene expression. These results demonstrate that WRKY41 may be a key regulator in the cross talk of salicylic acid and jasmonic acid pathways.
AB - Flagellin, a component of the flagellar filament of Pseudomonas syringae pv. tabaci 6605 (Pta), induces hypersensitive reaction in its non-host Arabidopsis thaliana. We identified the WRKY41 gene, which belongs to a multigene family encoding WRKY plant-specific transcription factors, as one of the flagellin-inducible genes in A. thaliana. Expression of WRKY41 is induced by inoculation with the incompatible pathogen P. syringae pv. tomato DC3000 (Pto) possessing AvrRpt2 and the non-host pathogens Pta within 6-h after inoculation, but not by inoculation with the compatible Pto. Expression of WRKY41 was also induced by inoculation of A. thaliana with an hrp-type three secretion system (T3SS)-defective mutant of Pto, indicating that effectors produced by T3SS in the Pto wild-type suppress the activation of WRKY41. Arabidopsis overexpressing WRKY41 showed enhanced resistance to the Pto wild-type but increased susceptibility to Erwinia carotovora EC1. WRKY41-overexpressing Arabidopsis constitutively expresses the PR5 gene, but suppresses the methyl jasmonate-induced PDF1.2 gene expression. These results demonstrate that WRKY41 may be a key regulator in the cross talk of salicylic acid and jasmonic acid pathways.
KW - FLS2
KW - Flagellin
KW - MAMP signaling pathway
KW - WRKY41
KW - flg22
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U2 - 10.1007/s00438-007-0315-0
DO - 10.1007/s00438-007-0315-0
M3 - Article
C2 - 18219494
AN - SCOPUS:39249084160
SN - 1617-4615
VL - 279
SP - 303
EP - 312
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 3
ER -