TY - JOUR
T1 - Molecular alterations of newly formed mandibular bone caused by zoledronate
AU - Yoshioka, Y.
AU - Yamachika, Eiki
AU - Nakanishi, M.
AU - Ninomiya, T.
AU - Nakatsuji, K.
AU - Matsubara, Masakazu
AU - Moritani, Norifumi
AU - Kobayashi, Y.
AU - Fujii, T.
AU - Iida, S.
N1 - Publisher Copyright:
© 2018 International Association of Oral and Maxillofacial Surgeons
PY - 2018/9
Y1 - 2018/9
N2 - Bone quality is defined by structural and material characteristics. Most studies on the mandible have focused on the analysis of structural characteristics, with insufficient investigation of material characteristics. This study tested whether zoledronate affects the material characteristics of newly formed mandibular bone. Thirty-six female Wistar rats were assigned to three groups: sham-ovariectomized rats (SHAM, n = 12), ovariectomized rats (OVX, n = 12), and ovariectomized rats treated with zoledronate (ZOL, n = 12). The left side of the mandibular ramus of all rats was drilled bicortically. Twenty-eight days after surgery, all surviving rats were euthanized and all mandibles were removed. Raman microspectroscopy was performed, and five spectra per specimen of newly formed mandibular bone were analysed. Compared with OVX rats, the mineral/matrix ratio in ZOL rats was significantly increased (5.43 ± 1.88 vs. 7.86 ± 2.05), while crystallinity (0.055 ± 0.002 vs. 0.050 ± 0.002), relative proteoglycan content (0.43 ± 0.10 vs. 0.31 ± 0.05), and collagen structural integrity (1.16 ± 0.21 vs. 0.72 ± 0.06) were significantly decreased. These changes in material characteristics may explain why rats that received zoledronate exhibited peculiar biological phenomena such as bisphosphonate-related osteonecrosis of the jaw.
AB - Bone quality is defined by structural and material characteristics. Most studies on the mandible have focused on the analysis of structural characteristics, with insufficient investigation of material characteristics. This study tested whether zoledronate affects the material characteristics of newly formed mandibular bone. Thirty-six female Wistar rats were assigned to three groups: sham-ovariectomized rats (SHAM, n = 12), ovariectomized rats (OVX, n = 12), and ovariectomized rats treated with zoledronate (ZOL, n = 12). The left side of the mandibular ramus of all rats was drilled bicortically. Twenty-eight days after surgery, all surviving rats were euthanized and all mandibles were removed. Raman microspectroscopy was performed, and five spectra per specimen of newly formed mandibular bone were analysed. Compared with OVX rats, the mineral/matrix ratio in ZOL rats was significantly increased (5.43 ± 1.88 vs. 7.86 ± 2.05), while crystallinity (0.055 ± 0.002 vs. 0.050 ± 0.002), relative proteoglycan content (0.43 ± 0.10 vs. 0.31 ± 0.05), and collagen structural integrity (1.16 ± 0.21 vs. 0.72 ± 0.06) were significantly decreased. These changes in material characteristics may explain why rats that received zoledronate exhibited peculiar biological phenomena such as bisphosphonate-related osteonecrosis of the jaw.
KW - Raman spectroscopy
KW - bisphosphonate
KW - bone quality
KW - mandible
KW - osteonecrosis
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U2 - 10.1016/j.ijom.2018.02.002
DO - 10.1016/j.ijom.2018.02.002
M3 - Article
C2 - 29550280
AN - SCOPUS:85043536816
SN - 0901-5027
VL - 47
SP - 1206
EP - 1213
JO - International Journal of Oral and Maxillofacial Surgery
JF - International Journal of Oral and Maxillofacial Surgery
IS - 9
ER -