Molecular analysis for bacterial contamination in dental unit water lines

Akari Watanabe, Naofumi Tamaki, Miwa Matsuyama, Susumu Kokeguchi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.

Original languageEnglish
Pages (from-to)143-145
Number of pages3
JournalNew Microbiologica
Issue number2
Publication statusPublished - Apr 2016


  • Bacterial contamination
  • Dental unit water line
  • Molecular analysis

ASJC Scopus subject areas

  • Microbiology (medical)


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