Molecular analysis for bacterial contamination in dental unit water lines

Akari Watanabe; Naofumi Tamaki; Miwa Matsuyama; Susumu Kokeguchi

Molecular analysis for bacterial contamination in dental unit water lines

Akari Watanabe, Naofumi Tamaki, Miwa Matsuyama, Susumu Kokeguchi

Research output: Contribution to journal › Article › peer-review

Abstract

Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.

Original language	English
Pages (from-to)	143-145
Number of pages	3
Journal	New Microbiologica
Volume	39
Issue number	2
Publication status	Published - Apr 2016

Keywords

Bacterial contamination
Dental unit water line
Molecular analysis
PCR-DGGE

ASJC Scopus subject areas

Microbiology (medical)

Cite this

@article{87d96b7684ec41fd819a00390d91fe6e,

title = "Molecular analysis for bacterial contamination in dental unit water lines",

abstract = "Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.",

keywords = "Bacterial contamination, Dental unit water line, Molecular analysis, PCR-DGGE",

author = "Akari Watanabe and Naofumi Tamaki and Miwa Matsuyama and Susumu Kokeguchi",

note = "Funding Information: This work was supported by Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) (KAKENHI Grant Number 26463163). The authors declare no conflicts of interest in the publication of this study. Publisher Copyright: {\textcopyright} 2016 by Edimes - Edizioni Internazionali Srl. All rights reserved.",

year = "2016",

month = apr,

language = "English",

volume = "39",

pages = "143--145",

journal = "New Microbiologica",

issn = "1121-7138",

publisher = "Luigi Ponzio e figlio Editori",

number = "2",

}

TY - JOUR

T1 - Molecular analysis for bacterial contamination in dental unit water lines

AU - Watanabe, Akari

AU - Tamaki, Naofumi

AU - Matsuyama, Miwa

AU - Kokeguchi, Susumu

N1 - Funding Information: This work was supported by Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) (KAKENHI Grant Number 26463163). The authors declare no conflicts of interest in the publication of this study. Publisher Copyright: © 2016 by Edimes - Edizioni Internazionali Srl. All rights reserved.

PY - 2016/4

Y1 - 2016/4

N2 - Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.

AB - Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.

KW - Bacterial contamination

KW - Dental unit water line

KW - Molecular analysis

KW - PCR-DGGE

UR - http://www.scopus.com/inward/record.url?scp=84971450149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84971450149&partnerID=8YFLogxK

M3 - Article

C2 - 27196554

AN - SCOPUS:84971450149

SN - 1121-7138

VL - 39

SP - 143

EP - 145

JO - New Microbiologica

JF - New Microbiologica

IS - 2

ER -

Molecular analysis for bacterial contamination in dental unit water lines

Abstract

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this