TY - JOUR
T1 - Molecular and structural patterns of bone regeneration in surgically created defects containing bone substitutes
AU - Elgali, Ibrahim
AU - Igawa, Kazuyo
AU - Palmquist, Anders
AU - Lennerås, Maria
AU - Xia, Wei
AU - Choi, Sungjin
AU - Chung, Ung Il
AU - Omar, Omar
AU - Thomsen, Peter
N1 - Funding Information:
The authors are indebted to Lena Emanuelsson and Birgitta Norlindh for excellent technical assistance during surgery and histological preparation. This study was supported by the Swedish Research Council ( K2012-52X-09495-25-3 ), the BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy , the IngaBritt and Arne Lundberg Foundation , ALF/LUA research grant , the Stiftelsen Handlanden Hjalmar Svensson , the Japan Society for the Promotion of Science (JSPS) through the Grants-in-Aid for Scientific Research, the International Core Research Center for Nanobio , Core-to-Core Program and the Japan Science and Technology Agency (JST) through the S-innovation program. IE is the recipient of an international scholarship for PhD studies provided by the Ministry of Higher Education, Libya . KI is the recipient of an international postdoc grant provided by BIOMATCELL and BIOSUM National Graduate School in Biomaterials and Cell Therapy . The grant providers were not involved in the study design, data acquisition, interpretation, writing and submission of the article.
PY - 2014/3
Y1 - 2014/3
N2 - Several biomaterials have been introduced for bone augmentation. However, information is lacking about the mechanisms of bone regeneration and/or integration of these materials in the recipient bone. This study aimed to determine the molecular and structural events in bone defects after augmentation with synthetic tetrapod-shaped calcium phosphate (Tetrabone; TetraB) compared with natural deproteinized bovine bone (DBB). Defects were created in the epiphyses of rat femurs and filled with TetraB or DBB or left empty (Sham). After 3, 6, 14 and 28 d, samples were harvested for histology, histomorphometry, ultrastructure and gene expression analyses. At 3 d, higher expressions of bone formation (ALP and OC) and remodeling (CatK) genes were detected in TetraB compared with DBB and Sham. Downregulation of bone remodeling genes (TRAP and CatK) was detected in DBB as compared to Sham after 14 d. Histomorphometry at 6 and 14 d demonstrated greater bone contact with the granules in TetraB. At 28 d, a larger bone area per defect was found in TetraB. The present experiments show that a synthetic substitute, consisting of α-tricalcium and octacalcium phosphates, induces early osteogenic and osteoclastic activities and promotes bone formation in trabecular bone defects.
AB - Several biomaterials have been introduced for bone augmentation. However, information is lacking about the mechanisms of bone regeneration and/or integration of these materials in the recipient bone. This study aimed to determine the molecular and structural events in bone defects after augmentation with synthetic tetrapod-shaped calcium phosphate (Tetrabone; TetraB) compared with natural deproteinized bovine bone (DBB). Defects were created in the epiphyses of rat femurs and filled with TetraB or DBB or left empty (Sham). After 3, 6, 14 and 28 d, samples were harvested for histology, histomorphometry, ultrastructure and gene expression analyses. At 3 d, higher expressions of bone formation (ALP and OC) and remodeling (CatK) genes were detected in TetraB compared with DBB and Sham. Downregulation of bone remodeling genes (TRAP and CatK) was detected in DBB as compared to Sham after 14 d. Histomorphometry at 6 and 14 d demonstrated greater bone contact with the granules in TetraB. At 28 d, a larger bone area per defect was found in TetraB. The present experiments show that a synthetic substitute, consisting of α-tricalcium and octacalcium phosphates, induces early osteogenic and osteoclastic activities and promotes bone formation in trabecular bone defects.
KW - Deproteinized bovine bone
KW - Gene expression
KW - In vivo
KW - Octacalcium phosphate
KW - Ultrastructure
KW - α-Tricalcium phosphate
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U2 - 10.1016/j.biomaterials.2013.12.084
DO - 10.1016/j.biomaterials.2013.12.084
M3 - Article
C2 - 24439408
AN - SCOPUS:84893644395
SN - 0142-9612
VL - 35
SP - 3229
EP - 3242
JO - Biomaterials
JF - Biomaterials
IS - 10
ER -