Molecular Cloning and Characterization of a cDNA Encoding Putative Phospholipid Hydroperoxide Glutathione Peroxidase from Spinach

Manabu Sugimoto; Satoshi Furui; Yukio Suzuki

doi:10.1271/bbb.61.1379

Molecular Cloning and Characterization of a cDNA Encoding Putative Phospholipid Hydroperoxide Glutathione Peroxidase from Spinach

Manabu Sugimoto, Satoshi Furui, Yukio Suzuki

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

A cDNA encoding spinach putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The cDNA included an open reading frame that encoded a polypeptide of 171 amino acid residues. The deduced amino acid sequence showed about 77 and 50% similarity to plant putative PHGPXs and mammalian PHGPXs, respectively. PCR products with the same size as that of the spinach putative PHGPX were obtained from maize, soybeans, and Arabidopsis, suggesting the expression of putative PHGPX genes in other plants.

Original language	English
Pages (from-to)	1379-1381
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	61
Issue number	8
DOIs	https://doi.org/10.1271/bbb.61.1379
Publication status	Published - Jan 1997

Keywords

Gene cloning
Nucleotide sequence
Phospholipid hydroperoxide glutathione peroxidase
Spinacia oleracea L

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Access to Document

10.1271/bbb.61.1379

Cite this

@article{0f85519562974f76a872f39f3cebce0d,

title = "Molecular Cloning and Characterization of a cDNA Encoding Putative Phospholipid Hydroperoxide Glutathione Peroxidase from Spinach",

abstract = "A cDNA encoding spinach putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The cDNA included an open reading frame that encoded a polypeptide of 171 amino acid residues. The deduced amino acid sequence showed about 77 and 50% similarity to plant putative PHGPXs and mammalian PHGPXs, respectively. PCR products with the same size as that of the spinach putative PHGPX were obtained from maize, soybeans, and Arabidopsis, suggesting the expression of putative PHGPX genes in other plants.",

keywords = "Gene cloning, Nucleotide sequence, Phospholipid hydroperoxide glutathione peroxidase, Spinacia oleracea L",

author = "Manabu Sugimoto and Satoshi Furui and Yukio Suzuki",

year = "1997",

month = jan,

doi = "10.1271/bbb.61.1379",

language = "English",

volume = "61",

pages = "1379--1381",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "8",

}

TY - JOUR

T1 - Molecular Cloning and Characterization of a cDNA Encoding Putative Phospholipid Hydroperoxide Glutathione Peroxidase from Spinach

AU - Sugimoto, Manabu

AU - Furui, Satoshi

AU - Suzuki, Yukio

PY - 1997/1

Y1 - 1997/1

N2 - A cDNA encoding spinach putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The cDNA included an open reading frame that encoded a polypeptide of 171 amino acid residues. The deduced amino acid sequence showed about 77 and 50% similarity to plant putative PHGPXs and mammalian PHGPXs, respectively. PCR products with the same size as that of the spinach putative PHGPX were obtained from maize, soybeans, and Arabidopsis, suggesting the expression of putative PHGPX genes in other plants.

AB - A cDNA encoding spinach putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The cDNA included an open reading frame that encoded a polypeptide of 171 amino acid residues. The deduced amino acid sequence showed about 77 and 50% similarity to plant putative PHGPXs and mammalian PHGPXs, respectively. PCR products with the same size as that of the spinach putative PHGPX were obtained from maize, soybeans, and Arabidopsis, suggesting the expression of putative PHGPX genes in other plants.

KW - Gene cloning

KW - Nucleotide sequence

KW - Phospholipid hydroperoxide glutathione peroxidase

KW - Spinacia oleracea L

UR - http://www.scopus.com/inward/record.url?scp=0031203609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031203609&partnerID=8YFLogxK

U2 - 10.1271/bbb.61.1379

DO - 10.1271/bbb.61.1379

M3 - Article

C2 - 9301122

AN - SCOPUS:0031203609

SN - 0916-8451

VL - 61

SP - 1379

EP - 1381

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 8

ER -

Molecular Cloning and Characterization of a cDNA Encoding Putative Phospholipid Hydroperoxide Glutathione Peroxidase from Spinach

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this