Abstract
Estrogens are essential for sex change and behavior in wrasses. To understand the molecular mechanisms of estrogen action in the reproduction of the protogynous wrasse, Halichoeres trimaculatus, we isolated cDNA encoding three estrogen receptor (ER) subtypes from ovary by 5' and 3' rapid amplification cDNAends (RACE) methods. The deduced amino acid sequence of ERα,ERβ1 and ERβ2 showed high identity to the red seabream ERa (81%), largemouth bass ERβ (82%) and largemouth bass ERy (81%), respectively. Real-time polymerase chain reaction revealed some distinctions in tissue expression of ERs, suggesting different functions for each ER subtype in protogynous wrasses.
| Original language | English |
|---|---|
| Pages (from-to) | 88-89 |
| Number of pages | 2 |
| Journal | Cybium |
| Volume | 32 |
| Issue number | 2 SUPPL. |
| Publication status | Published - Jul 31 2008 |
Keywords
- Cloning
- Estrogen receptor
- Expression
- Halichoeres trimaculatus
- Protogynous wrasse
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Animal Science and Zoology