TY - JOUR
T1 - Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours
AU - Ochiai, Kazuhiko
AU - Watanabe, Masami
AU - Azakami, Daigo
AU - Michishita, Masaki
AU - Yoshikawa, Yasunaga
AU - Udagawa, Chihiro
AU - Metheenukul, Pornphimon
AU - Chahomchuen, Thippayarat
AU - Aoki, Hiroshi
AU - Kumon, Hiromi
AU - Morimatsu, Masami
AU - Omi, Toshinori
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Young Scientists (B) (23780326 and 24780313) and Scientific Research (C) (23580399) from the Japan Society for the Promotion of Science and supported in part by the Strategic Research Base Development Program for Private Universities from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), 2008–2012.
PY - 2013/9
Y1 - 2013/9
N2 - REIC/Dkk-3, a member of the human Dickkopf (Dkk) family, plays a role as a suppressor of growth in several human cancers. In this study, the tumour suppression function of canine REIC/Dkk-3 was investigated. The full-length open reading frame of the canine REIC/Dkk-3 homologue was cloned and the tissue distribution of REIC/Dkk-3 mRNA was determined, along with the subcellular localisation of the REIC/Dkk-3 protein in canine cancer cell lines. Expression of REIC/Dkk-3 was lower in mammary gland tumours and in canine mammary carcinoma cell lines than in normal mammary gland tissue. Overexpression of REIC/Dkk-3 induced apoptosis in canine mammary carcinoma cell lines. These results show that expression of REIC/Dkk-3 is downregulated in canine mammary tumours and that one of the functions of this gene is induction of apoptosis.
AB - REIC/Dkk-3, a member of the human Dickkopf (Dkk) family, plays a role as a suppressor of growth in several human cancers. In this study, the tumour suppression function of canine REIC/Dkk-3 was investigated. The full-length open reading frame of the canine REIC/Dkk-3 homologue was cloned and the tissue distribution of REIC/Dkk-3 mRNA was determined, along with the subcellular localisation of the REIC/Dkk-3 protein in canine cancer cell lines. Expression of REIC/Dkk-3 was lower in mammary gland tumours and in canine mammary carcinoma cell lines than in normal mammary gland tissue. Overexpression of REIC/Dkk-3 induced apoptosis in canine mammary carcinoma cell lines. These results show that expression of REIC/Dkk-3 is downregulated in canine mammary tumours and that one of the functions of this gene is induction of apoptosis.
KW - Canine
KW - Mammary gland tumours
KW - REIC/Dkk-3
KW - Tumour suppressor gene
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U2 - 10.1016/j.tvjl.2013.04.024
DO - 10.1016/j.tvjl.2013.04.024
M3 - Article
C2 - 23732075
AN - SCOPUS:84884980385
SN - 1090-0233
VL - 197
SP - 769
EP - 775
JO - Veterinary Journal
JF - Veterinary Journal
IS - 3
ER -
