TY - JOUR
T1 - Molecular cloning of cDNA and analysis of expression of the gene for α-glucosidase from the hypopharyngeal gland of the honeybee Apis mellifera L.
AU - Ohashi, Kazuaki
AU - Sawata, Miyuki
AU - Takeuchi, Hideaki
AU - Natori, Shunji
AU - Kubo, Takeo
N1 - Funding Information:
This work was supported by Grant-in-Aid for Specifically Promoted Research from the Ministry of Education, Science and Culture of Japan, and a research grant from the Sumitomo Foundation.
PY - 1996/4/16
Y1 - 1996/4/16
N2 - Previously, we identified and purified an α-glucosidase with a molecular mass of 70 kDa from homogenate, of the hypopharyngeal gland of the older worker bee (forager bee) Apis mellifera L. Here, we isolated and sequenced a cDNA for the α-glucosidase. The cDNA encoded a protein consisting of 650 amino acids, which had high sequence identity with fruit fly and mosquito possible maltase gene products. RT-PCR showed that the gene was expressed specifically in the hypopharyngeal gland of the forager bee.
AB - Previously, we identified and purified an α-glucosidase with a molecular mass of 70 kDa from homogenate, of the hypopharyngeal gland of the older worker bee (forager bee) Apis mellifera L. Here, we isolated and sequenced a cDNA for the α-glucosidase. The cDNA encoded a protein consisting of 650 amino acids, which had high sequence identity with fruit fly and mosquito possible maltase gene products. RT-PCR showed that the gene was expressed specifically in the hypopharyngeal gland of the forager bee.
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U2 - 10.1006/bbrc.1996.0604
DO - 10.1006/bbrc.1996.0604
M3 - Article
C2 - 8619864
AN - SCOPUS:0029868220
SN - 0006-291X
VL - 221
SP - 380
EP - 385
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -