TY - JOUR
T1 - Molecular cloning of cDNA encoding the 16 KDa subunit of vacuolar H+-ATPase from mouse cerebellum
AU - Hanada, Hironori
AU - Hasebe, Masahisa
AU - Moriyama, Yoshinori
AU - Maeda, Masatomo
AU - Futai, Masamitsu
N1 - Funding Information:
We are grateful to Dr. T. Furuichi (National Institute of Basic Biology) and Dr. K. Mikoshiba (Osaka University) for providing us mouse cerebellum CDNA library. This research was supported in part by grants from the Ministry of Education, Science and Culture of Japan and Human Frontier Science Program.
PY - 1991/5/15
Y1 - 1991/5/15
N2 - cDNa for the 16 kDa subunit of vacuolar H+-ATPase was cloned from mouse cerebellum and sequenced. The deduced polypeptide (155 amino acid residues; molecular weight, 15,808) was highly hydrophobic and homologous to the subunits of bovine adrenal medulla, Torpedo marmorata electric lobe, Drosophila and yeast. Glu-139 (supposed to be essential for proton transport) was also conserved as the potential dicyclohexylcarbodiimide binding site. The subunit had four transmembrane segments: Segment II and IV were highly homologous and Glu-139 was located in Segment IV. The roles of the non-conserved regions are discussed.
AB - cDNa for the 16 kDa subunit of vacuolar H+-ATPase was cloned from mouse cerebellum and sequenced. The deduced polypeptide (155 amino acid residues; molecular weight, 15,808) was highly hydrophobic and homologous to the subunits of bovine adrenal medulla, Torpedo marmorata electric lobe, Drosophila and yeast. Glu-139 (supposed to be essential for proton transport) was also conserved as the potential dicyclohexylcarbodiimide binding site. The subunit had four transmembrane segments: Segment II and IV were highly homologous and Glu-139 was located in Segment IV. The roles of the non-conserved regions are discussed.
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U2 - 10.1016/0006-291X(91)90391-J
DO - 10.1016/0006-291X(91)90391-J
M3 - Article
C2 - 1828149
AN - SCOPUS:0025820672
SN - 0006-291X
VL - 176
SP - 1062
EP - 1067
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -