Molecular cloning of the fibroin light chain complementary DNA and its use in the study of the expression of the light chain gene in the posterior silk gland of Bombyx mori

K. Kimura; F. Oyama; H. Ueda; S. Mizuno; K. Shimura

doi:10.1007/BF01951711

Molecular cloning of the fibroin light chain complementary DNA and its use in the study of the expression of the light chain gene in the posterior silk gland of Bombyx mori

K. Kimura, F. Oyama, H. Ueda, S. Mizuno, K. Shimura

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Fibroin light chain (L-chain) mRNA (mol. wt 4.0×10⁵ daltons) was purified from the posterior silk gland of the silkworm, Bombyx mori (J-131 strain). Double-stranded complementary DNA was synthesized and inserted into the PstI site of pBR322 employing the oligo(dC)-oligo(dG) tailing method. Several recombinant plasmids containing the inserts of about 800 base pairs were isolated. Hybridization-translation assay demonstrated that these clones hybridized specifically with the fibroin L-chain mRNA. One of these clones (pLA23) was used as a probe to investigate relative concentrations of the fibroin L-chain gene and mRNA in the posterior silk glands at different stages of late larval development.

Original language	English
Pages (from-to)	1167-1171
Number of pages	5
Journal	Experientia
Volume	41
Issue number	9
DOIs	https://doi.org/10.1007/BF01951711
Publication status	Published - Sept 1 1985
Externally published	Yes

Keywords

Bombyx mori
Silkworm
complementary DNA
fibroin light chain
mRNA
molecular cloning
silk gland

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1007/BF01951711

Cite this

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title = "Molecular cloning of the fibroin light chain complementary DNA and its use in the study of the expression of the light chain gene in the posterior silk gland of Bombyx mori",

abstract = "Fibroin light chain (L-chain) mRNA (mol. wt 4.0×105 daltons) was purified from the posterior silk gland of the silkworm, Bombyx mori (J-131 strain). Double-stranded complementary DNA was synthesized and inserted into the PstI site of pBR322 employing the oligo(dC)-oligo(dG) tailing method. Several recombinant plasmids containing the inserts of about 800 base pairs were isolated. Hybridization-translation assay demonstrated that these clones hybridized specifically with the fibroin L-chain mRNA. One of these clones (pLA23) was used as a probe to investigate relative concentrations of the fibroin L-chain gene and mRNA in the posterior silk glands at different stages of late larval development.",

keywords = "Bombyx mori, Silkworm, complementary DNA, fibroin light chain, mRNA, molecular cloning, silk gland",

author = "K. Kimura and F. Oyama and H. Ueda and S. Mizuno and K. Shimura",

year = "1985",

month = sep,

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doi = "10.1007/BF01951711",

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volume = "41",

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T1 - Molecular cloning of the fibroin light chain complementary DNA and its use in the study of the expression of the light chain gene in the posterior silk gland of Bombyx mori

AU - Kimura, K.

AU - Oyama, F.

AU - Ueda, H.

AU - Mizuno, S.

AU - Shimura, K.

PY - 1985/9/1

Y1 - 1985/9/1

N2 - Fibroin light chain (L-chain) mRNA (mol. wt 4.0×105 daltons) was purified from the posterior silk gland of the silkworm, Bombyx mori (J-131 strain). Double-stranded complementary DNA was synthesized and inserted into the PstI site of pBR322 employing the oligo(dC)-oligo(dG) tailing method. Several recombinant plasmids containing the inserts of about 800 base pairs were isolated. Hybridization-translation assay demonstrated that these clones hybridized specifically with the fibroin L-chain mRNA. One of these clones (pLA23) was used as a probe to investigate relative concentrations of the fibroin L-chain gene and mRNA in the posterior silk glands at different stages of late larval development.

AB - Fibroin light chain (L-chain) mRNA (mol. wt 4.0×105 daltons) was purified from the posterior silk gland of the silkworm, Bombyx mori (J-131 strain). Double-stranded complementary DNA was synthesized and inserted into the PstI site of pBR322 employing the oligo(dC)-oligo(dG) tailing method. Several recombinant plasmids containing the inserts of about 800 base pairs were isolated. Hybridization-translation assay demonstrated that these clones hybridized specifically with the fibroin L-chain mRNA. One of these clones (pLA23) was used as a probe to investigate relative concentrations of the fibroin L-chain gene and mRNA in the posterior silk glands at different stages of late larval development.

KW - Bombyx mori

KW - Silkworm

KW - complementary DNA

KW - fibroin light chain

KW - mRNA

KW - molecular cloning

KW - silk gland

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AN - SCOPUS:0022360273

SN - 0014-4754

VL - 41

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JO - Experientia

JF - Experientia

IS - 9

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Molecular cloning of the fibroin light chain complementary DNA and its use in the study of the expression of the light chain gene in the posterior silk gland of Bombyx mori

Abstract

Keywords

ASJC Scopus subject areas

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