Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis

Nobuyuki Kato; Makoto Hijikata; Yuko Ootsuyama; Masanori Nakagawa; Showgo Ohkoshi; Takashi Sugimura; Kunitada Shimotohno

doi:10.1073/pnas.87.24.9524

Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis

Nobuyuki Kato, Makoto Hijikata, Yuko Ootsuyama, Masanori Nakagawa, Showgo Ohkoshi, Takashi Sugimura, Kunitada Shimotohno

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

323 Citations (Scopus)

Abstract

The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese patients with chronic hepatitis. HCV-J genome contains a long open reading frame that can encode a sequence of 3010 amino acid residues. Comparison of HCV-J with the American isolate of HCV showed 22.6% difference in nucleotide sequence and 15.1% difference in amino acid sequence. Thus HCV-J and the American isolate of HCV are probably different subtypes of HCV. The relationship of HCV-J with other animal RNA virus families and the putative organization of the HCV-J genome are discussed. (.

Original language	English
Pages (from-to)	9524-9528
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	87
Issue number	24
DOIs	https://doi.org/10.1073/pnas.87.24.9524
Publication status	Published - 1990

Keywords

Gene structure
Nucleotide sequence
RNA virus

ASJC Scopus subject areas

General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1073/pnas.87.24.9524

Cite this

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abstract = "The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese patients with chronic hepatitis. HCV-J genome contains a long open reading frame that can encode a sequence of 3010 amino acid residues. Comparison of HCV-J with the American isolate of HCV showed 22.6% difference in nucleotide sequence and 15.1% difference in amino acid sequence. Thus HCV-J and the American isolate of HCV are probably different subtypes of HCV. The relationship of HCV-J with other animal RNA virus families and the putative organization of the HCV-J genome are discussed. (.",

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T1 - Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis

AU - Kato, Nobuyuki

AU - Hijikata, Makoto

AU - Ootsuyama, Yuko

AU - Nakagawa, Masanori

AU - Ohkoshi, Showgo

AU - Sugimura, Takashi

AU - Shimotohno, Kunitada

PY - 1990

Y1 - 1990

N2 - The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese patients with chronic hepatitis. HCV-J genome contains a long open reading frame that can encode a sequence of 3010 amino acid residues. Comparison of HCV-J with the American isolate of HCV showed 22.6% difference in nucleotide sequence and 15.1% difference in amino acid sequence. Thus HCV-J and the American isolate of HCV are probably different subtypes of HCV. The relationship of HCV-J with other animal RNA virus families and the putative organization of the HCV-J genome are discussed. (.

AB - The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese patients with chronic hepatitis. HCV-J genome contains a long open reading frame that can encode a sequence of 3010 amino acid residues. Comparison of HCV-J with the American isolate of HCV showed 22.6% difference in nucleotide sequence and 15.1% difference in amino acid sequence. Thus HCV-J and the American isolate of HCV are probably different subtypes of HCV. The relationship of HCV-J with other animal RNA virus families and the putative organization of the HCV-J genome are discussed. (.

KW - Gene structure

KW - Nucleotide sequence

KW - RNA virus

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Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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