Molecular epidemiological studies of Vibrio cholerae in Bengal region

Sumio Shinoda; Tomoko Nakagawa; Nobuyuki Hirakawa; Shin Ichi Miyoshi; Eiji Arakawa; Thandavarayan Ramamurthy; B. Dutta; Shah M. Faruque; Gopinath Balakrish Nair

doi:10.4265/bio.13.1

Molecular epidemiological studies of Vibrio cholerae in Bengal region

Sumio Shinoda, Tomoko Nakagawa, Nobuyuki Hirakawa, Shin Ichi Miyoshi, Eiji Arakawa, Thandavarayan Ramamurthy, B. Dutta, Shah M. Faruque, Gopinath Balakrish Nair

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Vibrio cholerae isolates from environmental and clinical origins in the Bengal region in which epidemics of cholera break out periodically were analyzed with particular emphasis on the molecular epidemiological features. The presence of the virulence genes (ctxA, tcpA and toxR) in the isolates was analyzed by the PCR (polymerase chain reaction) method. PFGE (pulsed-field gel electrophoresis) was performed to determine the clonal relationships between the clinical and environmental strains. Antibiograms and O serovars of the isolates were also examined. O1 and O139 strains from both clinical and environmental sources were all positive for the three virulence genes while non-O1 /non-O139 strains from both sources were all negative for ctxA and tcpA but positive for toxR. PFGE patterns of recent isolates of O1 and O139 were similar in each serovar regardless of origin, suggesting a clonal relationship between the clinical and environmental strains, although comparison with past isolates or isolates from different geographical area showed some differences.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Biocontrol science
Volume	13
Issue number	1
DOIs	https://doi.org/10.4265/bio.13.1
Publication status	Published - Mar 2008

Keywords

Bengal region
Molecular epidemiology
Pulsed-field gel electrophoresis
Vibrio cholerae

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Public Health, Environmental and Occupational Health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4265/bio.13.1

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title = "Molecular epidemiological studies of Vibrio cholerae in Bengal region",

abstract = "Vibrio cholerae isolates from environmental and clinical origins in the Bengal region in which epidemics of cholera break out periodically were analyzed with particular emphasis on the molecular epidemiological features. The presence of the virulence genes (ctxA, tcpA and toxR) in the isolates was analyzed by the PCR (polymerase chain reaction) method. PFGE (pulsed-field gel electrophoresis) was performed to determine the clonal relationships between the clinical and environmental strains. Antibiograms and O serovars of the isolates were also examined. O1 and O139 strains from both clinical and environmental sources were all positive for the three virulence genes while non-O1 /non-O139 strains from both sources were all negative for ctxA and tcpA but positive for toxR. PFGE patterns of recent isolates of O1 and O139 were similar in each serovar regardless of origin, suggesting a clonal relationship between the clinical and environmental strains, although comparison with past isolates or isolates from different geographical area showed some differences.",

keywords = "Bengal region, Molecular epidemiology, Pulsed-field gel electrophoresis, Vibrio cholerae",

author = "Sumio Shinoda and Tomoko Nakagawa and Nobuyuki Hirakawa and Miyoshi, {Shin Ichi} and Eiji Arakawa and Thandavarayan Ramamurthy and B. Dutta and Faruque, {Shah M.} and Nair, {Gopinath Balakrish}",

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doi = "10.4265/bio.13.1",

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AU - Shinoda, Sumio

AU - Nakagawa, Tomoko

AU - Hirakawa, Nobuyuki

AU - Miyoshi, Shin Ichi

AU - Arakawa, Eiji

AU - Ramamurthy, Thandavarayan

AU - Dutta, B.

AU - Faruque, Shah M.

AU - Nair, Gopinath Balakrish

PY - 2008/3

Y1 - 2008/3

N2 - Vibrio cholerae isolates from environmental and clinical origins in the Bengal region in which epidemics of cholera break out periodically were analyzed with particular emphasis on the molecular epidemiological features. The presence of the virulence genes (ctxA, tcpA and toxR) in the isolates was analyzed by the PCR (polymerase chain reaction) method. PFGE (pulsed-field gel electrophoresis) was performed to determine the clonal relationships between the clinical and environmental strains. Antibiograms and O serovars of the isolates were also examined. O1 and O139 strains from both clinical and environmental sources were all positive for the three virulence genes while non-O1 /non-O139 strains from both sources were all negative for ctxA and tcpA but positive for toxR. PFGE patterns of recent isolates of O1 and O139 were similar in each serovar regardless of origin, suggesting a clonal relationship between the clinical and environmental strains, although comparison with past isolates or isolates from different geographical area showed some differences.

AB - Vibrio cholerae isolates from environmental and clinical origins in the Bengal region in which epidemics of cholera break out periodically were analyzed with particular emphasis on the molecular epidemiological features. The presence of the virulence genes (ctxA, tcpA and toxR) in the isolates was analyzed by the PCR (polymerase chain reaction) method. PFGE (pulsed-field gel electrophoresis) was performed to determine the clonal relationships between the clinical and environmental strains. Antibiograms and O serovars of the isolates were also examined. O1 and O139 strains from both clinical and environmental sources were all positive for the three virulence genes while non-O1 /non-O139 strains from both sources were all negative for ctxA and tcpA but positive for toxR. PFGE patterns of recent isolates of O1 and O139 were similar in each serovar regardless of origin, suggesting a clonal relationship between the clinical and environmental strains, although comparison with past isolates or isolates from different geographical area showed some differences.

KW - Bengal region

KW - Molecular epidemiology

KW - Pulsed-field gel electrophoresis

KW - Vibrio cholerae

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Molecular epidemiological studies of Vibrio cholerae in Bengal region

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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