Molecular Monitoring of the Main Changes in Bacterial Floral Diversity in the Gastrointestinal Tract of a Thoroughbred Foal with Catarrhal Enteritis by Using PCR-DGGE

Hidetoshi Morita; Fumihiko Nakajima; Masaru Murakami; Akihito Endo; Takehito Suzuki; Chiharu Shiratori; Yukio Kato; Alexandre T. Okatani; Hiroaki Akita; Toshio Masaoka

doi:10.1016/j.jevs.2006.11.011

Molecular Monitoring of the Main Changes in Bacterial Floral Diversity in the Gastrointestinal Tract of a Thoroughbred Foal with Catarrhal Enteritis by Using PCR-DGGE

Hidetoshi Morita, Fumihiko Nakajima, Masaru Murakami, Akihito Endo, Takehito Suzuki, Chiharu Shiratori, Yukio Kato, Alexandre T. Okatani, Hiroaki Akita, Toshio Masaoka

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

In this study, the main changes in bacterial floral diversity in the gastrointestinal tract of a Thoroughbred foal were monitored by using polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). The foal died of catarrhal enteritis of the cecum and large colon. Diarrheal feces and gastrointestinal contents were compared with normal feces. The closest relatives of the bacterium in the samples were Lactobacillus johnsonii (100% similarity), uncultured Bacteroides sp. (92.5% similarity), Bacteroides fragilis (96.3% similarity), and Enterococcus faecium/Enterococcus durans (100% similarity); these were detected by PCR-DGGE using a universal primer set. Monitoring revealed that the numbers of Escherichia coli/Shigella sonnei (97.9% similarity) were significantly higher in the diarrheal feces. Thus, PCR-DGGE is a useful tool for monitoring the main changes in bacterial floral diversity occurring in the gastrointestinal tracts of Thoroughbreds.

Original language	English
Pages (from-to)	14-19
Number of pages	6
Journal	Journal of Equine Veterinary Science
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/j.jevs.2006.11.011
Publication status	Published - Jan 2007
Externally published	Yes

Keywords

Denaturing gradient gel electrophoresis (DGGE)
Gastrointestinal tract
Thoroughbred foal

ASJC Scopus subject areas

Equine

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10.1016/j.jevs.2006.11.011

Cite this

Morita, H., Nakajima, F., Murakami, M., Endo, A., Suzuki, T., Shiratori, C., Kato, Y., Okatani, A. T., Akita, H., & Masaoka, T. (2007). Molecular Monitoring of the Main Changes in Bacterial Floral Diversity in the Gastrointestinal Tract of a Thoroughbred Foal with Catarrhal Enteritis by Using PCR-DGGE. Journal of Equine Veterinary Science, 27(1), 14-19. https://doi.org/10.1016/j.jevs.2006.11.011

Molecular Monitoring of the Main Changes in Bacterial Floral Diversity in the Gastrointestinal Tract of a Thoroughbred Foal with Catarrhal Enteritis by Using PCR-DGGE. / Morita, Hidetoshi; Nakajima, Fumihiko; Murakami, Masaru et al.
In: Journal of Equine Veterinary Science, Vol. 27, No. 1, 01.2007, p. 14-19.

Research output: Contribution to journal › Article › peer-review

Morita, H, Nakajima, F, Murakami, M, Endo, A, Suzuki, T, Shiratori, C, Kato, Y, Okatani, AT, Akita, H & Masaoka, T 2007, 'Molecular Monitoring of the Main Changes in Bacterial Floral Diversity in the Gastrointestinal Tract of a Thoroughbred Foal with Catarrhal Enteritis by Using PCR-DGGE', Journal of Equine Veterinary Science, vol. 27, no. 1, pp. 14-19. https://doi.org/10.1016/j.jevs.2006.11.011

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abstract = "In this study, the main changes in bacterial floral diversity in the gastrointestinal tract of a Thoroughbred foal were monitored by using polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). The foal died of catarrhal enteritis of the cecum and large colon. Diarrheal feces and gastrointestinal contents were compared with normal feces. The closest relatives of the bacterium in the samples were Lactobacillus johnsonii (100% similarity), uncultured Bacteroides sp. (92.5% similarity), Bacteroides fragilis (96.3% similarity), and Enterococcus faecium/Enterococcus durans (100% similarity); these were detected by PCR-DGGE using a universal primer set. Monitoring revealed that the numbers of Escherichia coli/Shigella sonnei (97.9% similarity) were significantly higher in the diarrheal feces. Thus, PCR-DGGE is a useful tool for monitoring the main changes in bacterial floral diversity occurring in the gastrointestinal tracts of Thoroughbreds.",

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AU - Shiratori, Chiharu

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AU - Akita, Hiroaki

AU - Masaoka, Toshio

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AB - In this study, the main changes in bacterial floral diversity in the gastrointestinal tract of a Thoroughbred foal were monitored by using polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). The foal died of catarrhal enteritis of the cecum and large colon. Diarrheal feces and gastrointestinal contents were compared with normal feces. The closest relatives of the bacterium in the samples were Lactobacillus johnsonii (100% similarity), uncultured Bacteroides sp. (92.5% similarity), Bacteroides fragilis (96.3% similarity), and Enterococcus faecium/Enterococcus durans (100% similarity); these were detected by PCR-DGGE using a universal primer set. Monitoring revealed that the numbers of Escherichia coli/Shigella sonnei (97.9% similarity) were significantly higher in the diarrheal feces. Thus, PCR-DGGE is a useful tool for monitoring the main changes in bacterial floral diversity occurring in the gastrointestinal tracts of Thoroughbreds.

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Molecular Monitoring of the Main Changes in Bacterial Floral Diversity in the Gastrointestinal Tract of a Thoroughbred Foal with Catarrhal Enteritis by Using PCR-DGGE

Abstract

Keywords

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