Molecular sieve in rat tubular basement membrane as revealed by negative staining

Hirofumi Makino, Zensuke Ota, Yasumasa Takaya, Akira Miyoshi, Tadashi Ofuji

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Rat tubuli were isolated both by the method of Krisko and by a modified method of Cook and Pickering; renal tubular basement membrane (TBM) was isolated by sonic disruption and by the method of Carlson. Using electron microscopy after negative staining, TBM of rat kidney isolated under these different conditions was shown to be a fine meshwork. Strands of the meshwork were interwoven, apparently enclosing pores to make up whole basement membrane. These findings are compared with our previous observation that glomerular and alveolar basement membranes were made up of a similarly fine meshwork.

Original languageEnglish
Pages (from-to)180-190
Number of pages11
JournalKidney and Blood Pressure Research
Issue number4
Publication statusPublished - Jan 1 1981


  • Molecular sieve
  • Negative staining Electron microscopy
  • Tubular basement membrane
  • Ultrastructure

ASJC Scopus subject areas

  • Nephrology
  • Cardiology and Cardiovascular Medicine


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