Molecular targeting for epidermal growth factor receptor expressed on breast cancer cells by human fusion protein

Masakazu Ueda; Kyriakos Psarras; Hiromitsu Jinno; Tadashi Ikeda; Kohji Enomoto; Masaki Kitajima; Junichiro Futami; Hidenori Yamada; Masaharu Seno

doi:10.1007/BF02966516

Molecular targeting for epidermal growth factor receptor expressed on breast cancer cells by human fusion protein

Masakazu Ueda, Kyriakos Psarras, Hiromitsu Jinno, Tadashi Ikeda, Kohji Enomoto, Masaki Kitajima, Junichiro Futami, Hidenori Yamada, Masaharu Seno

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Recombinant human ribonuclease 1 (RNasel) was chemically linked to recombinant human epidermal growth factor (EGF). The cytotoxicity of this conjugate was assayed using MTT assay. The EGF-RNase conjugate showed dose-dependent cytotoxicity against breast and squamous cell carcinomas overexpressing the EGF receptor (EGFR). The cytotoxicity of the conjugate correlated positively with the level of EGFR expression by each cell line. These results suggest that the EGF-RNase conjugate is a more effective anticancer agent with less immunogenicity and toxicity than conventional chimeric breast cancer toxins.

Original language	English
Pages (from-to)	253-255
Number of pages	3
Journal	Breast Cancer
Volume	4
Issue number	4
DOIs	https://doi.org/10.1007/BF02966516
Publication status	Published - Dec 1997

Keywords

Breast cancer
EGFR
Fusion protein
RNase
Targeting

ASJC Scopus subject areas

Oncology
Radiology Nuclear Medicine and imaging
Pharmacology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF02966516

Cite this

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title = "Molecular targeting for epidermal growth factor receptor expressed on breast cancer cells by human fusion protein",

abstract = "Recombinant human ribonuclease 1 (RNasel) was chemically linked to recombinant human epidermal growth factor (EGF). The cytotoxicity of this conjugate was assayed using MTT assay. The EGF-RNase conjugate showed dose-dependent cytotoxicity against breast and squamous cell carcinomas overexpressing the EGF receptor (EGFR). The cytotoxicity of the conjugate correlated positively with the level of EGFR expression by each cell line. These results suggest that the EGF-RNase conjugate is a more effective anticancer agent with less immunogenicity and toxicity than conventional chimeric breast cancer toxins.",

keywords = "Breast cancer, EGFR, Fusion protein, RNase, Targeting",

author = "Masakazu Ueda and Kyriakos Psarras and Hiromitsu Jinno and Tadashi Ikeda and Kohji Enomoto and Masaki Kitajima and Junichiro Futami and Hidenori Yamada and Masaharu Seno",

year = "1997",

month = dec,

doi = "10.1007/BF02966516",

language = "English",

volume = "4",

pages = "253--255",

journal = "Breast Cancer",

issn = "1340-6868",

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TY - JOUR

T1 - Molecular targeting for epidermal growth factor receptor expressed on breast cancer cells by human fusion protein

AU - Ueda, Masakazu

AU - Psarras, Kyriakos

AU - Jinno, Hiromitsu

AU - Ikeda, Tadashi

AU - Enomoto, Kohji

AU - Kitajima, Masaki

AU - Futami, Junichiro

AU - Yamada, Hidenori

AU - Seno, Masaharu

PY - 1997/12

Y1 - 1997/12

N2 - Recombinant human ribonuclease 1 (RNasel) was chemically linked to recombinant human epidermal growth factor (EGF). The cytotoxicity of this conjugate was assayed using MTT assay. The EGF-RNase conjugate showed dose-dependent cytotoxicity against breast and squamous cell carcinomas overexpressing the EGF receptor (EGFR). The cytotoxicity of the conjugate correlated positively with the level of EGFR expression by each cell line. These results suggest that the EGF-RNase conjugate is a more effective anticancer agent with less immunogenicity and toxicity than conventional chimeric breast cancer toxins.

AB - Recombinant human ribonuclease 1 (RNasel) was chemically linked to recombinant human epidermal growth factor (EGF). The cytotoxicity of this conjugate was assayed using MTT assay. The EGF-RNase conjugate showed dose-dependent cytotoxicity against breast and squamous cell carcinomas overexpressing the EGF receptor (EGFR). The cytotoxicity of the conjugate correlated positively with the level of EGFR expression by each cell line. These results suggest that the EGF-RNase conjugate is a more effective anticancer agent with less immunogenicity and toxicity than conventional chimeric breast cancer toxins.

KW - Breast cancer

KW - EGFR

KW - Fusion protein

KW - RNase

KW - Targeting

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DO - 10.1007/BF02966516

M3 - Article

AN - SCOPUS:0001620356

SN - 1340-6868

VL - 4

SP - 253

EP - 255

JO - Breast Cancer

JF - Breast Cancer

IS - 4

ER -

Molecular targeting for epidermal growth factor receptor expressed on breast cancer cells by human fusion protein

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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