Monitoring of bacterial contamination of dental unit water lines using adenosine triphosphate bioluminescence

A. Watanabe; N. Tamaki; K. Yokota; M. Matsuyama; S. Kokeguchi

doi:10.1016/j.jhin.2016.08.001

Monitoring of bacterial contamination of dental unit water lines using adenosine triphosphate bioluminescence

A. Watanabe, N. Tamaki, K. Yokota, M. Matsuyama, S. Kokeguchi

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Bacterial contamination of dental unit waterlines (DUWLs) was evaluated using ATP bioluminescence analysis and a conventional culture method. Water samples (N = 44) from DUWLs were investigated for heterotrophic bacteria by culture on R2A agar, which gave counts ranging from 1.4×10³ to 2.7×10⁵ cfu/mL. The ATP bioluminescence results for DUWL samples ranged from 6 to 1189 relative light units and could be obtained within 1 min; these correlated well with the culture results (r = 0.727–0.855). We conclude that the results of the ATP bioluminescence assay accurately reflect the results of conventional culture-based testing. This method is potentially useful for rapid and simple monitoring of DUWL bacterial contamination.

Original language	English
Pages (from-to)	393-396
Number of pages	4
Journal	Journal of Hospital Infection
Volume	94
Issue number	4
DOIs	https://doi.org/10.1016/j.jhin.2016.08.001
Publication status	Published - Dec 1 2016

Keywords

ATP bioluminescence
Bacteria
Contamination
Dental unit water line
Infection control

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jhin.2016.08.001

Cite this

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title = "Monitoring of bacterial contamination of dental unit water lines using adenosine triphosphate bioluminescence",

abstract = "Bacterial contamination of dental unit waterlines (DUWLs) was evaluated using ATP bioluminescence analysis and a conventional culture method. Water samples (N = 44) from DUWLs were investigated for heterotrophic bacteria by culture on R2A agar, which gave counts ranging from 1.4×103 to 2.7×105 cfu/mL. The ATP bioluminescence results for DUWL samples ranged from 6 to 1189 relative light units and could be obtained within 1 min; these correlated well with the culture results (r = 0.727–0.855). We conclude that the results of the ATP bioluminescence assay accurately reflect the results of conventional culture-based testing. This method is potentially useful for rapid and simple monitoring of DUWL bacterial contamination.",

keywords = "ATP bioluminescence, Bacteria, Contamination, Dental unit water line, Infection control",

author = "A. Watanabe and N. Tamaki and K. Yokota and M. Matsuyama and S. Kokeguchi",

note = "Funding Information: This work was supported by Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) (KAKENHI Grant Number 26463163 ). Publisher Copyright: {\textcopyright} 2016 The Healthcare Infection Society",

year = "2016",

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doi = "10.1016/j.jhin.2016.08.001",

language = "English",

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TY - JOUR

T1 - Monitoring of bacterial contamination of dental unit water lines using adenosine triphosphate bioluminescence

AU - Watanabe, A.

AU - Tamaki, N.

AU - Yokota, K.

AU - Matsuyama, M.

AU - Kokeguchi, S.

N1 - Funding Information: This work was supported by Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) (KAKENHI Grant Number 26463163 ). Publisher Copyright: © 2016 The Healthcare Infection Society

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Bacterial contamination of dental unit waterlines (DUWLs) was evaluated using ATP bioluminescence analysis and a conventional culture method. Water samples (N = 44) from DUWLs were investigated for heterotrophic bacteria by culture on R2A agar, which gave counts ranging from 1.4×103 to 2.7×105 cfu/mL. The ATP bioluminescence results for DUWL samples ranged from 6 to 1189 relative light units and could be obtained within 1 min; these correlated well with the culture results (r = 0.727–0.855). We conclude that the results of the ATP bioluminescence assay accurately reflect the results of conventional culture-based testing. This method is potentially useful for rapid and simple monitoring of DUWL bacterial contamination.

AB - Bacterial contamination of dental unit waterlines (DUWLs) was evaluated using ATP bioluminescence analysis and a conventional culture method. Water samples (N = 44) from DUWLs were investigated for heterotrophic bacteria by culture on R2A agar, which gave counts ranging from 1.4×103 to 2.7×105 cfu/mL. The ATP bioluminescence results for DUWL samples ranged from 6 to 1189 relative light units and could be obtained within 1 min; these correlated well with the culture results (r = 0.727–0.855). We conclude that the results of the ATP bioluminescence assay accurately reflect the results of conventional culture-based testing. This method is potentially useful for rapid and simple monitoring of DUWL bacterial contamination.

KW - ATP bioluminescence

KW - Bacteria

KW - Contamination

KW - Dental unit water line

KW - Infection control

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DO - 10.1016/j.jhin.2016.08.001

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SN - 0195-6701

VL - 94

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JO - Journal of Hospital Infection

JF - Journal of Hospital Infection

IS - 4

ER -

Monitoring of bacterial contamination of dental unit water lines using adenosine triphosphate bioluminescence

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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