TY - JOUR
T1 - Morphological and genetic analysis of three bacteriophages of Serratia marcescens isolated from environmental water
T2 - Research Letter
AU - Matsushita, Kenshi
AU - Uchiyama, Jumpei
AU - Kato, Shin Ichiro
AU - Ujihara, Takako
AU - Hoshiba, Hiroshi
AU - Sugihara, Shigeyoshi
AU - Muraoka, Asako
AU - Wakiguchi, Hiroshi
AU - Matsuzaki, Shigenobu
PY - 2009/2
Y1 - 2009/2
N2 - Increases in multidrug-resistant strains of Serratia marcescens are of great concern in pediatrics, especially in neonatal intensive care units. In the search for bacteriophages to control infectious diseases caused by multidrug-resistant S. marcescens, three phages (KSP20, KSP90, and KSP100) were isolated from environmental water and were characterized morphologically and genetically. KSP20 and KSP90 belonged to morphotype A1 of the family Myoviridae, and KSP100 belonged to morphotype C3 of the family Podoviridae. Analysis of the DNA region coding virion proteins, together with their morphological features, indicated that KSP20, KSP90, and KSP100 were related to the P2-like phage (temperate), T4-type phage (virulent), and phiEco32 phage (virulent), respectively. Based on amino acid sequences of the major capsid protein, KSP90 formed a new branch with a Stenotrophomonas maltophilia phage, Smp14, in the T4-type phage phylogeny. Both Smp14 and phiEco32 have been reported as potential therapeutic phages. These results suggest that KSP90 and KSP100 may be candidate therapeutic phages to control S. marcescens infection.
AB - Increases in multidrug-resistant strains of Serratia marcescens are of great concern in pediatrics, especially in neonatal intensive care units. In the search for bacteriophages to control infectious diseases caused by multidrug-resistant S. marcescens, three phages (KSP20, KSP90, and KSP100) were isolated from environmental water and were characterized morphologically and genetically. KSP20 and KSP90 belonged to morphotype A1 of the family Myoviridae, and KSP100 belonged to morphotype C3 of the family Podoviridae. Analysis of the DNA region coding virion proteins, together with their morphological features, indicated that KSP20, KSP90, and KSP100 were related to the P2-like phage (temperate), T4-type phage (virulent), and phiEco32 phage (virulent), respectively. Based on amino acid sequences of the major capsid protein, KSP90 formed a new branch with a Stenotrophomonas maltophilia phage, Smp14, in the T4-type phage phylogeny. Both Smp14 and phiEco32 have been reported as potential therapeutic phages. These results suggest that KSP90 and KSP100 may be candidate therapeutic phages to control S. marcescens infection.
KW - P2-like phage
KW - Phage therapy
KW - PhiEco32-like phage
KW - Serratia marcescens
KW - T4-type phage
UR - http://www.scopus.com/inward/record.url?scp=58449111642&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58449111642&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.2008.01455.x
DO - 10.1111/j.1574-6968.2008.01455.x
M3 - Article
C2 - 19087204
AN - SCOPUS:58449111642
SN - 0378-1097
VL - 291
SP - 201
EP - 208
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -
