TY - JOUR
T1 - Mouse mandible contains distinctive mesenchymal stem cells
AU - Yamaza, T.
AU - Ren, G.
AU - Akiyama, K.
AU - Chen, C.
AU - Shi, Y.
AU - Shi, S.
N1 - Funding Information:
The authors thank Dr. Larry Fisher for providing antibodies for this study. This work was supported by grants from the National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services (ARRA 1R01DE019413 to S.S. and Y.S., and R01DE017449 to S.S.), National Institutes of Health (AI057596 to Y.S.), and New Jersey Commission on Science and Technology (NJCST-2042–014–84 to Y.S.).
PY - 2011/3
Y1 - 2011/3
N2 - Although human orofacial bone-marrow-derived mesenchymal stem cells showed differentiation traits distinctly different from those of mesenchymal stem cells (MSCs) derived from long bone marrow (BMMSCs), mouse MSCs derived from orofacial bone have not been isolated due to technical difficulties, which in turn precludes the use of mouse models to study and cure orofacial diseases. In this study, we developed techniques to isolate and expand mouse orofacial bone/bone-marrow-derived MSCs (OMSCs) from mandibles and verified their MSC characteristics by single-colony formation, multi-lineage differentiation, and in vivo tissue regeneration. Activated T-lymphocytes impaired OMSCs via the Fas/Fas ligand pathway, as occurs in BMMSCs. Furthermore, we found that OMSCs are distinct from BMMSCs with respect to regulating T-lymphocyte survival and proliferation. Analysis of our data suggests that OMSCs are a unique population of MSCs and play an important role in systemic immunity. Abbreviations: BMMSC, bone marrow mesenchymal stem cell; HA/TCP, hydroxyapatite/tricalcium phosphate; OMSC, orofacial mesenchymal stem cell; OVX, ovariectomized.
AB - Although human orofacial bone-marrow-derived mesenchymal stem cells showed differentiation traits distinctly different from those of mesenchymal stem cells (MSCs) derived from long bone marrow (BMMSCs), mouse MSCs derived from orofacial bone have not been isolated due to technical difficulties, which in turn precludes the use of mouse models to study and cure orofacial diseases. In this study, we developed techniques to isolate and expand mouse orofacial bone/bone-marrow-derived MSCs (OMSCs) from mandibles and verified their MSC characteristics by single-colony formation, multi-lineage differentiation, and in vivo tissue regeneration. Activated T-lymphocytes impaired OMSCs via the Fas/Fas ligand pathway, as occurs in BMMSCs. Furthermore, we found that OMSCs are distinct from BMMSCs with respect to regulating T-lymphocyte survival and proliferation. Analysis of our data suggests that OMSCs are a unique population of MSCs and play an important role in systemic immunity. Abbreviations: BMMSC, bone marrow mesenchymal stem cell; HA/TCP, hydroxyapatite/tricalcium phosphate; OMSC, orofacial mesenchymal stem cell; OVX, ovariectomized.
KW - differentiation
KW - mesenchymal stem cells
KW - mouse mandible
KW - tissue regeneration
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U2 - 10.1177/0022034510387796
DO - 10.1177/0022034510387796
M3 - Article
C2 - 21076121
AN - SCOPUS:79952063283
SN - 0022-0345
VL - 90
SP - 317
EP - 324
JO - Journal of dental research
JF - Journal of dental research
IS - 3
ER -
