TY - JOUR
T1 - MPK9 and MPK12 function in SA-induced stomatal closure in arabidopsis thaliana
AU - Khokon, Md Atiqur Rahman
AU - Salam, Mohammad Abdus
AU - Jammes, Fabien
AU - Ye, Wenxiu
AU - Hossain, Mohammad Anowar
AU - Okuma, Eiji
AU - Nakamura, Yoshimasa
AU - Mori, Izumi C.
AU - Kwak, June M.
AU - Murata, Yoshiyuki
N1 - Funding Information:
This research was supported in part by The Asahi Glass Foundation, NOVARTIS Foundation (Japan) for the Promotion of Science, Grant-in-Aid for Young Scientists, and Grants for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan to Y.M., and in part by BS-R013-G2 from the institute of Basic Science to J.M.K.
Publisher Copyright:
© 2017 Japan Society for Bioscience, Biotechnology, and Agrochemistry
PY - 2017
Y1 - 2017
N2 - Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) and methyl jasmonate (MeJA) signaling. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling and MeJA signaling in Arabidopsis thaliana. In this study, we examined whether these two MAPKs are involved in SA-induced stomatal closure using genetic mutants and a pharmacological, MAPKK inhibitor. Salicylic acid induced stomatal closure in mpk9 and mpk12 single mutants but not in mpk9 mpk12 double mutants. The MAPKK inhibitor PD98059 inhibited SA-induced stomatal closure in wild-type plants. Salicylic acid induced extracellular reactive oxygen species (ROS) production, intracellular ROS accumulation, and cytosolic alkalization in the mpk9, mpk12, and mpk9 mpk12 mutants. Moreover, SA-activated S-type anion channels in guard cells of wild-type plants but not in guard cells of mpk9 mpk12 double mutants. These results imply that MPK9 and MPK12 are positive regulators of SA signaling in Arabidopsis guard cells.
AB - Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) and methyl jasmonate (MeJA) signaling. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling and MeJA signaling in Arabidopsis thaliana. In this study, we examined whether these two MAPKs are involved in SA-induced stomatal closure using genetic mutants and a pharmacological, MAPKK inhibitor. Salicylic acid induced stomatal closure in mpk9 and mpk12 single mutants but not in mpk9 mpk12 double mutants. The MAPKK inhibitor PD98059 inhibited SA-induced stomatal closure in wild-type plants. Salicylic acid induced extracellular reactive oxygen species (ROS) production, intracellular ROS accumulation, and cytosolic alkalization in the mpk9, mpk12, and mpk9 mpk12 mutants. Moreover, SA-activated S-type anion channels in guard cells of wild-type plants but not in guard cells of mpk9 mpk12 double mutants. These results imply that MPK9 and MPK12 are positive regulators of SA signaling in Arabidopsis guard cells.
KW - Cytosolic alkalization
KW - Mitogen-activated protein kinase
KW - Reactive oxygen species
KW - S-type anion channel in guard cells
KW - Salicylic acid
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U2 - 10.1080/09168451.2017.1308244
DO - 10.1080/09168451.2017.1308244
M3 - Article
C2 - 28387156
AN - SCOPUS:85021175779
SN - 0916-8451
VL - 81
SP - 1394
EP - 1400
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 7
ER -