TY - JOUR
T1 - Multiple expression of glucose transporters in the lateral wall of the cochlear duct studied by quantitative real-time PCR assay
AU - Edamatsu, Midori
AU - Kondo, Yasuhiro
AU - Ando, Motonori
N1 - Funding Information:
The authors thank Dr. Shunji Takeuchi for his critical comments. This work was supported by a grant-in-aid for scientific research ( 22570064 ) from Japan Society for the Promotion of Science , and by a Ryobi Teien Memory Foundation grant.
PY - 2011/2/18
Y1 - 2011/2/18
N2 - We have investigated the gene expression of the facilitated glucose transporter (GLUT), H+-coupled myo-inositol cotransporter (HMIT), and Na+ glucose cotransporter (SGLT) in the lateral wall of the cochlear duct by conventional RT-PCR and quantitative real-time PCR. The isoforms GLUT1, -3, -4, -5, -8, -10, -12 and HMIT were detected in both the stria vascularis and the spiral ligament, whereas no SGLT isoforms could be detected in these tissues. Quantitative real-time PCR analysis revealed significant differences in the gene expression of GLUT1, -4, -5, -10, and HMIT isoforms between the stria vascularis and the spiral ligament. This result reflects the tissue-dependent distributions of GLUT isoforms. These findings strongly suggest that a number of GLUT isoforms participate in glucose transport in the stria vascularis and the spiral ligament.
AB - We have investigated the gene expression of the facilitated glucose transporter (GLUT), H+-coupled myo-inositol cotransporter (HMIT), and Na+ glucose cotransporter (SGLT) in the lateral wall of the cochlear duct by conventional RT-PCR and quantitative real-time PCR. The isoforms GLUT1, -3, -4, -5, -8, -10, -12 and HMIT were detected in both the stria vascularis and the spiral ligament, whereas no SGLT isoforms could be detected in these tissues. Quantitative real-time PCR analysis revealed significant differences in the gene expression of GLUT1, -4, -5, -10, and HMIT isoforms between the stria vascularis and the spiral ligament. This result reflects the tissue-dependent distributions of GLUT isoforms. These findings strongly suggest that a number of GLUT isoforms participate in glucose transport in the stria vascularis and the spiral ligament.
KW - GLUT
KW - Inner ear
KW - SLC2A
KW - Spiral ligament
KW - Stria vascularis
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U2 - 10.1016/j.neulet.2010.12.029
DO - 10.1016/j.neulet.2010.12.029
M3 - Article
C2 - 21182893
AN - SCOPUS:78751703459
SN - 0304-3940
VL - 490
SP - 72
EP - 77
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -