Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4+ and CD8+ T cells to nasal killed-bacillus calmette-guerin in mice

Hiroki Takahashi; Keiko Sasaki; Miyuki Takahashi; Naoko Shigemori; Shyunichi Honda; Hideyuki Arimitsu; Sadayuki Ochi; Naoya Ohara; Takao Tsuji

doi:10.1016/j.vaccine.2006.01.060

Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4⁺ and CD8⁺ T cells to nasal killed-bacillus calmette-guerin in mice

Hiroki Takahashi, Keiko Sasaki, Miyuki Takahashi, Naoko Shigemori, Shyunichi Honda, Hideyuki Arimitsu, Sadayuki Ochi, Naoya Ohara, Takao Tsuji

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

On single nasal immunization of mice with killed-bacillus calmette-guerin (BCG) plus a mutant Escherichia coli enterotoxin, delayed-type hypersensitivity was induced and BCG-infection decreased. Spleen cells, particularly CD4 ⁺ T cells among them produced IL-2, IFNγ and TNFα in response to the killed-BCG or purified protein derivatives. CD8⁺ T cells including cytotoxic T lymphocytes produced IFNγ and TNFα. However, both types of T cells reacted a little to Ag85B. The mutant induces cellular immunity to nasal killed-BCG vaccine and decreases BCG-infection. CD4⁺ and CD8⁺ T cells produce cytokines effective for tuberculosis. Although killed-BCG loses some antigens like Ag85B, nasal killed-BCG plus the mutant is useful for tuberculosis.

Original language	English
Pages (from-to)	3591-3598
Number of pages	8
Journal	Vaccine
Volume	24
Issue number	17
DOIs	https://doi.org/10.1016/j.vaccine.2006.01.060
Publication status	Published - Apr 24 2006
Externally published	Yes

Keywords

Heat-labile enterotoxin
Killed-BCG
Mucosal adjuvant
Mutant

ASJC Scopus subject areas

Molecular Medicine
Immunology and Microbiology(all)
veterinary(all)
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.vaccine.2006.01.060

Cite this

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title = "Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4+ and CD8+ T cells to nasal killed-bacillus calmette-guerin in mice",

abstract = "On single nasal immunization of mice with killed-bacillus calmette-guerin (BCG) plus a mutant Escherichia coli enterotoxin, delayed-type hypersensitivity was induced and BCG-infection decreased. Spleen cells, particularly CD4 + T cells among them produced IL-2, IFNγ and TNFα in response to the killed-BCG or purified protein derivatives. CD8+ T cells including cytotoxic T lymphocytes produced IFNγ and TNFα. However, both types of T cells reacted a little to Ag85B. The mutant induces cellular immunity to nasal killed-BCG vaccine and decreases BCG-infection. CD4+ and CD8+ T cells produce cytokines effective for tuberculosis. Although killed-BCG loses some antigens like Ag85B, nasal killed-BCG plus the mutant is useful for tuberculosis.",

keywords = "Heat-labile enterotoxin, Killed-BCG, Mucosal adjuvant, Mutant",

author = "Hiroki Takahashi and Keiko Sasaki and Miyuki Takahashi and Naoko Shigemori and Shyunichi Honda and Hideyuki Arimitsu and Sadayuki Ochi and Naoya Ohara and Takao Tsuji",

note = "Funding Information: This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan, and for the Open Research Center's Project of Fujita Health University from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",

year = "2006",

month = apr,

day = "24",

doi = "10.1016/j.vaccine.2006.01.060",

language = "English",

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T1 - Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4+ and CD8+ T cells to nasal killed-bacillus calmette-guerin in mice

AU - Takahashi, Hiroki

AU - Sasaki, Keiko

AU - Takahashi, Miyuki

AU - Shigemori, Naoko

AU - Honda, Shyunichi

AU - Arimitsu, Hideyuki

AU - Ochi, Sadayuki

AU - Ohara, Naoya

AU - Tsuji, Takao

N1 - Funding Information: This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan, and for the Open Research Center's Project of Fujita Health University from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

PY - 2006/4/24

Y1 - 2006/4/24

N2 - On single nasal immunization of mice with killed-bacillus calmette-guerin (BCG) plus a mutant Escherichia coli enterotoxin, delayed-type hypersensitivity was induced and BCG-infection decreased. Spleen cells, particularly CD4 + T cells among them produced IL-2, IFNγ and TNFα in response to the killed-BCG or purified protein derivatives. CD8+ T cells including cytotoxic T lymphocytes produced IFNγ and TNFα. However, both types of T cells reacted a little to Ag85B. The mutant induces cellular immunity to nasal killed-BCG vaccine and decreases BCG-infection. CD4+ and CD8+ T cells produce cytokines effective for tuberculosis. Although killed-BCG loses some antigens like Ag85B, nasal killed-BCG plus the mutant is useful for tuberculosis.

AB - On single nasal immunization of mice with killed-bacillus calmette-guerin (BCG) plus a mutant Escherichia coli enterotoxin, delayed-type hypersensitivity was induced and BCG-infection decreased. Spleen cells, particularly CD4 + T cells among them produced IL-2, IFNγ and TNFα in response to the killed-BCG or purified protein derivatives. CD8+ T cells including cytotoxic T lymphocytes produced IFNγ and TNFα. However, both types of T cells reacted a little to Ag85B. The mutant induces cellular immunity to nasal killed-BCG vaccine and decreases BCG-infection. CD4+ and CD8+ T cells produce cytokines effective for tuberculosis. Although killed-BCG loses some antigens like Ag85B, nasal killed-BCG plus the mutant is useful for tuberculosis.

KW - Heat-labile enterotoxin

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KW - Mucosal adjuvant

KW - Mutant

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