TY - JOUR
T1 - Mutation and DNA modification in Salmonella exposed to N-nitrosodimethylamine under UVA- and sunlight-irradiation
AU - Arimoto-Kobayashi, Sakae
AU - Tracey, Beryl M.
AU - Asao, Mieko
AU - Hayatsu, Hikoya
AU - Farmer, Peter B.
N1 - Funding Information:
This work was supported by the Grant-in-Aid for International Scientific Research (10044290) and Grant-in-Aid for Scientific Research (11672228) from the Ministry of Education, Science, Sports and Culture, Japan. The authors wish to thank Dr. G.M.A. Sweetman (Medical Research Council, Toxicology Unit, Leicester, UK) for his technical supports.
PY - 1999/8/18
Y1 - 1999/8/18
N2 - Previously, we reported that when Salmonella typhimurium and Escherichia coli were treated with N-nitrosodimethylamine (NDMA) under irradiation with ultraviolet-A (UVA), mutagenesis of the bacteria took place without externally added activation enzymes. We also observed the formation of O6-methylguanine (O6-meG), N7-methylguanine (N7-meG) and 7,8-dihydro-8-oxodeoxyguanosine (8-oxodG) in calf thymus DNA treated with NDMA plus UVA. In this study, we observed the mutagenicity of NDMA under irradiation of natural sunlight in S. typhimurium. Furthermore, we detected the formation of O6-meG, N7-meG and 8-oxodG in calf thymus DNA treated with NDMA plus simulated sunlight. Regarding the mutagenesis of S. typhimurium by NDMA plus UVA, we have now identified and quantified O6-meG formed in the genomic DNA of the bacteria under conditions of the mutagenesis. Copyright (C) 1999 Elsevier Science B.V.
AB - Previously, we reported that when Salmonella typhimurium and Escherichia coli were treated with N-nitrosodimethylamine (NDMA) under irradiation with ultraviolet-A (UVA), mutagenesis of the bacteria took place without externally added activation enzymes. We also observed the formation of O6-methylguanine (O6-meG), N7-methylguanine (N7-meG) and 7,8-dihydro-8-oxodeoxyguanosine (8-oxodG) in calf thymus DNA treated with NDMA plus UVA. In this study, we observed the mutagenicity of NDMA under irradiation of natural sunlight in S. typhimurium. Furthermore, we detected the formation of O6-meG, N7-meG and 8-oxodG in calf thymus DNA treated with NDMA plus simulated sunlight. Regarding the mutagenesis of S. typhimurium by NDMA plus UVA, we have now identified and quantified O6-meG formed in the genomic DNA of the bacteria under conditions of the mutagenesis. Copyright (C) 1999 Elsevier Science B.V.
KW - 7,8-Dihydro-8-oxo-deoxyguanosine
KW - Mutation
KW - N-Nitrosodimethylamine
KW - O-Methylguanine
KW - Sunlight
KW - UVA
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U2 - 10.1016/S1383-5718(99)00113-8
DO - 10.1016/S1383-5718(99)00113-8
M3 - Article
C2 - 10521681
AN - SCOPUS:0032872483
SN - 1383-5718
VL - 444
SP - 413
EP - 419
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
IS - 2
ER -