Mutation of the key residue for extraribosomal function of ribosomal protein S19 cause increased grooming behaviors in mice

Jun Chen; Taku Kaitsuka; Rika Fujino; Kimi Araki; Kazuhito Tomizawa; Tetsuro Yamamoto

doi:10.1016/j.neulet.2016.07.022

Mutation of the key residue for extraribosomal function of ribosomal protein S19 cause increased grooming behaviors in mice

Jun Chen, Taku Kaitsuka, Rika Fujino, Kimi Araki, Kazuhito Tomizawa, Tetsuro Yamamoto

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Ribosomal protein S19 (RP S19) possesses ribosomal function as RP S19 monomer and extraribosomal function as cross-linked RP S19 oligomers which function as a ligand of the complement 5a (C5a) receptor (CD88). We have generated a Gln137Glu-RP S19 knock-in (KI) mouse, which is shown to possess the weakened extraribosomal function of RP S19. Because whether the extraribosomal function of RP S19 has a role in brain function had been unclear, we performed behavioral analysis on these mice and demonstrated that KI mice displayed an increased grooming behavior during open-field test and elevated plus maze test and an enhanced freezing behavior in contextual fear conditioning test. These results suggest an involvement of RP S19 oligomers in some anxiety-like behavior, especially grooming behavior.

Original language	English
Pages (from-to)	221-226
Number of pages	6
Journal	Neuroscience Letters
Volume	629
DOIs	https://doi.org/10.1016/j.neulet.2016.07.022
Publication status	Published - Aug 26 2016

Keywords

Anxiety-like behavior
Complement system
Extraribosomal function
Fear memory
Grooming behavior
Ribosomal protein S19

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/j.neulet.2016.07.022

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@article{26888ea89e9340c3b089eedc43febfea,

title = "Mutation of the key residue for extraribosomal function of ribosomal protein S19 cause increased grooming behaviors in mice",

abstract = "Ribosomal protein S19 (RP S19) possesses ribosomal function as RP S19 monomer and extraribosomal function as cross-linked RP S19 oligomers which function as a ligand of the complement 5a (C5a) receptor (CD88). We have generated a Gln137Glu-RP S19 knock-in (KI) mouse, which is shown to possess the weakened extraribosomal function of RP S19. Because whether the extraribosomal function of RP S19 has a role in brain function had been unclear, we performed behavioral analysis on these mice and demonstrated that KI mice displayed an increased grooming behavior during open-field test and elevated plus maze test and an enhanced freezing behavior in contextual fear conditioning test. These results suggest an involvement of RP S19 oligomers in some anxiety-like behavior, especially grooming behavior.",

keywords = "Anxiety-like behavior, Complement system, Extraribosomal function, Fear memory, Grooming behavior, Ribosomal protein S19",

author = "Jun Chen and Taku Kaitsuka and Rika Fujino and Kimi Araki and Kazuhito Tomizawa and Tetsuro Yamamoto",

note = "Funding Information: This work was supported by a Grant-in Aid for Scientific Research C ( KAKENHI 24590484 to T. Y.) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan , by A-STEP ( AS251Z01945Q to T. Y.) from Japan Science and Technology Agency , by a Grant-in-Aid for Young Scientists B ( KAKENHI 15K18971 to T. K.) from the Japan Society for the Promotion of Science and by a Grant-in-Aid for Scientific Research from the {\textquoteleft}1-10 sen-nin-kai{\textquoteright} of an alumni association, School of Pharmacy, Kumamoto university . Publisher Copyright: {\textcopyright} 2016 Elsevier Ireland Ltd",

year = "2016",

month = aug,

day = "26",

doi = "10.1016/j.neulet.2016.07.022",

language = "English",

volume = "629",

pages = "221--226",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

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TY - JOUR

T1 - Mutation of the key residue for extraribosomal function of ribosomal protein S19 cause increased grooming behaviors in mice

AU - Chen, Jun

AU - Kaitsuka, Taku

AU - Fujino, Rika

AU - Araki, Kimi

AU - Tomizawa, Kazuhito

AU - Yamamoto, Tetsuro

N1 - Funding Information: This work was supported by a Grant-in Aid for Scientific Research C ( KAKENHI 24590484 to T. Y.) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan , by A-STEP ( AS251Z01945Q to T. Y.) from Japan Science and Technology Agency , by a Grant-in-Aid for Young Scientists B ( KAKENHI 15K18971 to T. K.) from the Japan Society for the Promotion of Science and by a Grant-in-Aid for Scientific Research from the ‘1-10 sen-nin-kai’ of an alumni association, School of Pharmacy, Kumamoto university . Publisher Copyright: © 2016 Elsevier Ireland Ltd

PY - 2016/8/26

Y1 - 2016/8/26

N2 - Ribosomal protein S19 (RP S19) possesses ribosomal function as RP S19 monomer and extraribosomal function as cross-linked RP S19 oligomers which function as a ligand of the complement 5a (C5a) receptor (CD88). We have generated a Gln137Glu-RP S19 knock-in (KI) mouse, which is shown to possess the weakened extraribosomal function of RP S19. Because whether the extraribosomal function of RP S19 has a role in brain function had been unclear, we performed behavioral analysis on these mice and demonstrated that KI mice displayed an increased grooming behavior during open-field test and elevated plus maze test and an enhanced freezing behavior in contextual fear conditioning test. These results suggest an involvement of RP S19 oligomers in some anxiety-like behavior, especially grooming behavior.

AB - Ribosomal protein S19 (RP S19) possesses ribosomal function as RP S19 monomer and extraribosomal function as cross-linked RP S19 oligomers which function as a ligand of the complement 5a (C5a) receptor (CD88). We have generated a Gln137Glu-RP S19 knock-in (KI) mouse, which is shown to possess the weakened extraribosomal function of RP S19. Because whether the extraribosomal function of RP S19 has a role in brain function had been unclear, we performed behavioral analysis on these mice and demonstrated that KI mice displayed an increased grooming behavior during open-field test and elevated plus maze test and an enhanced freezing behavior in contextual fear conditioning test. These results suggest an involvement of RP S19 oligomers in some anxiety-like behavior, especially grooming behavior.

KW - Anxiety-like behavior

KW - Complement system

KW - Extraribosomal function

KW - Fear memory

KW - Grooming behavior

KW - Ribosomal protein S19

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JO - Neuroscience Letters

JF - Neuroscience Letters

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Mutation of the key residue for extraribosomal function of ribosomal protein S19 cause increased grooming behaviors in mice

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Keywords

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