Mutation spectrum resulting in M13mp2 phage DNA exposed to N-nitrosoproline with UVA irradiation

Yumi Horai; Yoshiko Ando; Sachiko Kimura; Sakae Arimoto-Kobayashi

doi:10.1016/j.mrgentox.2017.06.003

Mutation spectrum resulting in M13mp2 phage DNA exposed to N-nitrosoproline with UVA irradiation

Yumi Horai, Yoshiko Ando, Sachiko Kimura, Sakae Arimoto-Kobayashi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

N-nitrosoproline (NPRO) is endogenously formed from proline and nitrite. In an effort to delineate the mechanism of NPRO-induced photomutagenicity, we investigated the mutagenic spectrum of NPRO on M13mp2 DNA with UVA irradiation. Following exposure to NPRO and UVA, the mutation frequency increased significantly in an NPRO and UVA dose-dependent manner. The sequence data derived from seventy of the mutants indicated that mutagenesis resulted mainly from an increase in single-base substitutions, the most frequent being GC to CG transversions. Non-clustering of the GC to CG mutations suggests that NPRO + UVA damage to DNA is random. These transversions may be caused by guanine adducts in DNA or in part by oxidatively modified guanine in DNA exposed to NPRO and UVA.

Original language	English
Pages (from-to)	1-4
Number of pages	4
Journal	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume	821
DOIs	https://doi.org/10.1016/j.mrgentox.2017.06.003
Publication status	Published - Sept 2017

Keywords

Mutation frequencies
N-nitrosamine
Oxidative damage
Photomutagenicity
UVA

ASJC Scopus subject areas

Genetics
Health, Toxicology and Mutagenesis

Access to Document

10.1016/j.mrgentox.2017.06.003

Cite this

@article{a1625e6c443e43a38ade34c533acefc5,

title = "Mutation spectrum resulting in M13mp2 phage DNA exposed to N-nitrosoproline with UVA irradiation",

abstract = "N-nitrosoproline (NPRO) is endogenously formed from proline and nitrite. In an effort to delineate the mechanism of NPRO-induced photomutagenicity, we investigated the mutagenic spectrum of NPRO on M13mp2 DNA with UVA irradiation. Following exposure to NPRO and UVA, the mutation frequency increased significantly in an NPRO and UVA dose-dependent manner. The sequence data derived from seventy of the mutants indicated that mutagenesis resulted mainly from an increase in single-base substitutions, the most frequent being GC to CG transversions. Non-clustering of the GC to CG mutations suggests that NPRO + UVA damage to DNA is random. These transversions may be caused by guanine adducts in DNA or in part by oxidatively modified guanine in DNA exposed to NPRO and UVA.",

keywords = "Mutation frequencies, N-nitrosamine, Oxidative damage, Photomutagenicity, UVA",

author = "Yumi Horai and Yoshiko Ando and Sachiko Kimura and Sakae Arimoto-Kobayashi",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

year = "2017",

month = sep,

doi = "10.1016/j.mrgentox.2017.06.003",

language = "English",

volume = "821",

pages = "1--4",

journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",

issn = "1383-5718",

publisher = "Elsevier",

}

TY - JOUR

T1 - Mutation spectrum resulting in M13mp2 phage DNA exposed to N-nitrosoproline with UVA irradiation

AU - Horai, Yumi

AU - Ando, Yoshiko

AU - Kimura, Sachiko

AU - Arimoto-Kobayashi, Sakae

PY - 2017/9

Y1 - 2017/9

N2 - N-nitrosoproline (NPRO) is endogenously formed from proline and nitrite. In an effort to delineate the mechanism of NPRO-induced photomutagenicity, we investigated the mutagenic spectrum of NPRO on M13mp2 DNA with UVA irradiation. Following exposure to NPRO and UVA, the mutation frequency increased significantly in an NPRO and UVA dose-dependent manner. The sequence data derived from seventy of the mutants indicated that mutagenesis resulted mainly from an increase in single-base substitutions, the most frequent being GC to CG transversions. Non-clustering of the GC to CG mutations suggests that NPRO + UVA damage to DNA is random. These transversions may be caused by guanine adducts in DNA or in part by oxidatively modified guanine in DNA exposed to NPRO and UVA.

AB - N-nitrosoproline (NPRO) is endogenously formed from proline and nitrite. In an effort to delineate the mechanism of NPRO-induced photomutagenicity, we investigated the mutagenic spectrum of NPRO on M13mp2 DNA with UVA irradiation. Following exposure to NPRO and UVA, the mutation frequency increased significantly in an NPRO and UVA dose-dependent manner. The sequence data derived from seventy of the mutants indicated that mutagenesis resulted mainly from an increase in single-base substitutions, the most frequent being GC to CG transversions. Non-clustering of the GC to CG mutations suggests that NPRO + UVA damage to DNA is random. These transversions may be caused by guanine adducts in DNA or in part by oxidatively modified guanine in DNA exposed to NPRO and UVA.

KW - Mutation frequencies

KW - N-nitrosamine

KW - Oxidative damage

KW - Photomutagenicity

KW - UVA

UR - http://www.scopus.com/inward/record.url?scp=85021640045&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021640045&partnerID=8YFLogxK

U2 - 10.1016/j.mrgentox.2017.06.003

DO - 10.1016/j.mrgentox.2017.06.003

M3 - Article

C2 - 28735738

AN - SCOPUS:85021640045

SN - 1383-5718

VL - 821

SP - 1

EP - 4

JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

ER -

Mutation spectrum resulting in M13mp2 phage DNA exposed to N-nitrosoproline with UVA irradiation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this