Mutations in Ran system affected telomere silencing in Saccharomyces cerevisiae

Naoyuki Hayashi, Masahiko Kobayashi, Hiroko Shimizu, Ken ichi Yamamoto, Seishi Murakami, Takeharu Nishimoto

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


The Ran GTPase system regulates the direction and timing of several cellular events, such as nuclear-cytosolic transport, centrosome formation, and nuclear envelope assembly in telophase. To gain insight into the Ran system's involvement in chromatin formation, we investigated gene silencing at the telomere in several mutants of the budding yeast Saccharomyces cerevisiae, which had defects in genes involved in the Ran system. A mutation of the RanGAP gene, rna1-1, caused reduced silencing at the telomere, and partial disruption of the nuclear Ran binding factor, yrb2-Δ2, increased this silencing. The reduced telomere silencing in rna1-1 cells was suppressed by a high dosage of the SIR3 gene or the SIT4 gene. Furthermore, hyperphosphorylated Sir3 protein accumulated in the rna1-1 mutant. These results suggest that RanGAP is required for the heterochromatin structure at the telomere in budding yeast.

Original languageEnglish
Pages (from-to)788-794
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - Nov 23 2007
Externally publishedYes


  • Budding yeast
  • Gsp1
  • Heterochromatin
  • Ran
  • Rna1
  • Sir3
  • Sit4
  • Snf2
  • Telomere silencing
  • Yrb2

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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