N-Linked sugar chain of 55-kda royal jelly glycoprotein

Yoshinobu Kimura; Shin Ichiro Kajiyama; Jun Kanaeda; Tomomi Izukawa; Masami Yonekura

doi:10.1271/bbb.60.2099

N-Linked sugar chain of 55-kda royal jelly glycoprotein

Yoshinobu Kimura, Shin Ichiro Kajiyama, Jun Kanaeda, Tomomi Izukawa, Masami Yonekura

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507–509 (1995)], has been identified. The sugar chains were released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential exo-mannosidase digestions, MALDI-TOF MS, and 500 MHz ¹H-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Manα1→2Manα1→6(Manα1→2Manα1→3)Manαl→6(Manα1→2Manα1→2Manα1 →3)Manβ1→4GlcNAcβl→4GlcNAc, which is a non-processed high mannose type structure.

Original language	English
Pages (from-to)	2099-2102
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	60
Issue number	12
DOIs	https://doi.org/10.1271/bbb.60.2099
Publication status	Published - Jan 1996

Keywords

Insect glycoprotein
MALDI-TOF MS
N-glycan;
PA-sugar chain
Royal jelly

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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@article{cd184e448ed64021bc7084be3a8c5e6e,

title = "N-Linked sugar chain of 55-kda royal jelly glycoprotein",

abstract = "An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507–509 (1995)], has been identified. The sugar chains were released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential exo-mannosidase digestions, MALDI-TOF MS, and 500 MHz 1H-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Manα1→2Manα1→6(Manα1→2Manα1→3)Manαl→6(Manα1→2Manα1→2Manα1 →3)Manβ1→4GlcNAcβl→4GlcNAc, which is a non-processed high mannose type structure.",

keywords = "Insect glycoprotein, MALDI-TOF MS, N-glycan;, PA-sugar chain, Royal jelly",

author = "Yoshinobu Kimura and Kajiyama, {Shin Ichiro} and Jun Kanaeda and Tomomi Izukawa and Masami Yonekura",

note = "Funding Information: t This work was supported in part by a Grant-in-Aid for Scientific Research [No. 07660442 (1995-1996)] from the Ministry of Education, Science, and Culture of Japan. tt Present address: Department of Biotechnology, Faculty of Engineering, Osaka University, Suita, Osaka 565, Japan. ttt To whom all correspondence should be addressed. Abbreviations: PA-, pyridylamino; TFA, trifluoroacetic acid; RP-, reversed phase; SF-, size-fractionation; RJGP, royal jelly glycoprotein; Man, D-mannose; GlcNAc, N-acetyl-D-glucosamine; MALDI-TOF. matrix-assisted laser desorption/ionization-time of flight; M9A, Man~I--+2Man~l--+ 6(ManlXl--+2ManlXl--+3)ManlXl--+6(ManlXl--+2ManlXl--+2Manod--+3)Manpl--+4GlcNAcPI--+4GlcNAc; MI. Manpl--+4GlcNacpt --+4GlcNAc; GN2, GlcNAcPl--+4GlcNAc-PA.",

year = "1996",

month = jan,

doi = "10.1271/bbb.60.2099",

language = "English",

volume = "60",

pages = "2099--2102",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "12",

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T1 - N-Linked sugar chain of 55-kda royal jelly glycoprotein

AU - Kimura, Yoshinobu

AU - Kajiyama, Shin Ichiro

AU - Kanaeda, Jun

AU - Izukawa, Tomomi

AU - Yonekura, Masami

N1 - Funding Information: t This work was supported in part by a Grant-in-Aid for Scientific Research [No. 07660442 (1995-1996)] from the Ministry of Education, Science, and Culture of Japan. tt Present address: Department of Biotechnology, Faculty of Engineering, Osaka University, Suita, Osaka 565, Japan. ttt To whom all correspondence should be addressed. Abbreviations: PA-, pyridylamino; TFA, trifluoroacetic acid; RP-, reversed phase; SF-, size-fractionation; RJGP, royal jelly glycoprotein; Man, D-mannose; GlcNAc, N-acetyl-D-glucosamine; MALDI-TOF. matrix-assisted laser desorption/ionization-time of flight; M9A, Man~I--+2Man~l--+ 6(ManlXl--+2ManlXl--+3)ManlXl--+6(ManlXl--+2ManlXl--+2Manod--+3)Manpl--+4GlcNAcPI--+4GlcNAc; MI. Manpl--+4GlcNacpt --+4GlcNAc; GN2, GlcNAcPl--+4GlcNAc-PA.

PY - 1996/1

Y1 - 1996/1

N2 - An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507–509 (1995)], has been identified. The sugar chains were released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential exo-mannosidase digestions, MALDI-TOF MS, and 500 MHz 1H-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Manα1→2Manα1→6(Manα1→2Manα1→3)Manαl→6(Manα1→2Manα1→2Manα1 →3)Manβ1→4GlcNAcβl→4GlcNAc, which is a non-processed high mannose type structure.

AB - An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507–509 (1995)], has been identified. The sugar chains were released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential exo-mannosidase digestions, MALDI-TOF MS, and 500 MHz 1H-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Manα1→2Manα1→6(Manα1→2Manα1→3)Manαl→6(Manα1→2Manα1→2Manα1 →3)Manβ1→4GlcNAcβl→4GlcNAc, which is a non-processed high mannose type structure.

KW - Insect glycoprotein

KW - MALDI-TOF MS

KW - N-glycan;

KW - PA-sugar chain

KW - Royal jelly

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DO - 10.1271/bbb.60.2099

M3 - Article

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AN - SCOPUS:0030433572

SN - 0916-8451

VL - 60

SP - 2099

EP - 2102

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 12

ER -

N-Linked sugar chain of 55-kda royal jelly glycoprotein

Abstract

Keywords

ASJC Scopus subject areas

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