N-Linked Sugar Chains of 350-kDa Royal Jelly Glycoprotein

Yoshinobu Kimura, Noriyuki Washino, Masami Yonekura

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40 Citations (Scopus)


The structures of N-linked sugar chains from 350 kDa royal jelly glycoprotein (RJG), which stimulates the proliferation of human monocytes, have been analyzed. The structural analyses of the N-linked sugar chains released by hydrazinolysis were done by two-dimensional sugar mapping of the corresponding pyridylamino derivatives and 500MHz 1H-NMR. All four structures were found to fall into the category of oligomannose-type sugar chains as follows; Manα 1→6 (Manα 1→3)Manα 1→6 (Manα 1→3) Manβ 1→4 GlcNAcβ 1→4 GlcNAc, Manα 1→2 Manα 1→6 (Manα 1→3) Manα 1→6 (Manα 1→2 Manα 1→3) Manβ 1 →4 GlcNAcβ 1 →4 GlcNAc, Manα 1→2Manα 1→6 (Manα 1→3) Manα 1→6 (Manα 1 →2 Manα 1→2Manα 1→3) Manβ 1→4 GlcNAcβ 1→4 GlcNAc, and Manα 1→2 Manα 1→6(Manα 1→2 Manα 1→3) Manα 1→6 (Manα 1→2 Manα 1→2 Manα 1→3) Manβ 1→4 GlcNAcβ 1→4 GlcNAc.

Original languageEnglish
Pages (from-to)507-509
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Issue number3
Publication statusPublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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