TY - GEN
T1 - Nanofluidic enzyme reactor exceeding limit of bulk reaction rate
AU - Yamamoto, Koki
AU - Morikawa, Kyojiro
AU - Imamura, Koreyoshi
AU - Imanaka, Hiroyuki
AU - Kitamori, Takehiko
N1 - Funding Information:
This study was financially supported by JST, CREST. Grant number: JPMJCR14G1.
PY - 2019
Y1 - 2019
N2 - We development a nanofluidic enzyme reactor exceeding the limitation of bulk enzyme reaction. The limit concentration of bulk trypsin solution is approximately 0.5 PM because self-digestion occurs over 0.5 PM solution, which complicates analysis of target proteins by digestion trypsin itself. Using extremely high surface/volume ration of nanospace, trypsin concentration, which indicates number of immobilized trypsin molecules on the nanochannel surface for liquid phase volume, exceed to bulk limit of concentration. As a result, 31 times higher concentration and 33 times faster enzyme reaction compared to bulk was realized. This reactor will be powerful tools which overcomes limitation in bulk and microfluidic method.
AB - We development a nanofluidic enzyme reactor exceeding the limitation of bulk enzyme reaction. The limit concentration of bulk trypsin solution is approximately 0.5 PM because self-digestion occurs over 0.5 PM solution, which complicates analysis of target proteins by digestion trypsin itself. Using extremely high surface/volume ration of nanospace, trypsin concentration, which indicates number of immobilized trypsin molecules on the nanochannel surface for liquid phase volume, exceed to bulk limit of concentration. As a result, 31 times higher concentration and 33 times faster enzyme reaction compared to bulk was realized. This reactor will be powerful tools which overcomes limitation in bulk and microfluidic method.
KW - Enzyme reactor
KW - Nanofluidics
KW - Single cell analysis
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=85094938345&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85094938345&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85094938345
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 113
EP - 114
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -