TY - JOUR
T1 - NB-LRR-encoding genes conferring susceptibility to organophosphate pesticides in sorghum
AU - Jing, Zihuan
AU - Wacera W, Fiona
AU - Takami, Tsuneaki
AU - Takanashi, Hideki
AU - Fukada, Fumi
AU - Kawano, Yoji
AU - Kajiya-Kanegae, Hiromi
AU - Iwata, Hiroyoshi
AU - Tsutsumi, Nobuhiro
AU - Sakamoto, Wataru
N1 - Funding Information:
We thank Makoto Ishii, Masaru Fujimoto, Rie Hijiya, Norikazu Ohnishi, and Adhiambo Everlyne Omollo for their technical assistance and discussions in performing this study. This work was partly supported by KAKENHI grants (17H03699, 18K19343, and 21H02508 to W.S.) from the Japan Society for the Promotion of Science (JSPS). We also thank the Oohara Foundation for financial support of our research group.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Organophosphate is the commonly used pesticide to control pest outbreak, such as those by aphids in many crops. Despite its wide use, however, necrotic lesion and/or cell death following the application of organophosphate pesticides has been reported to occur in several species. To understand this phenomenon, called organophosphate pesticide sensitivity (OPS) in sorghum, we conducted QTL analysis in a recombinant inbred line derived from the Japanese cultivar NOG, which exhibits OPS. Mapping OPS in this population identified a prominent QTL on chromosome 5, which corresponded to Organophosphate-Sensitive Reaction (OSR) reported previously in other mapping populations. The OSR locus included a cluster of three genes potentially encoding nucleotide-binding leucine-rich repeat (NB-LRR, NLR) proteins, among which NLR-C was considered to be responsible for OPS in a dominant fashion. NLR-C was functional in NOG, whereas the other resistant parent, BTx623, had a null mutation caused by the deletion of promoter sequences. Our finding of OSR as a dominant trait is important not only in understanding the diversified role of NB-LRR proteins in cereals but also in securing sorghum breeding free from OPS.
AB - Organophosphate is the commonly used pesticide to control pest outbreak, such as those by aphids in many crops. Despite its wide use, however, necrotic lesion and/or cell death following the application of organophosphate pesticides has been reported to occur in several species. To understand this phenomenon, called organophosphate pesticide sensitivity (OPS) in sorghum, we conducted QTL analysis in a recombinant inbred line derived from the Japanese cultivar NOG, which exhibits OPS. Mapping OPS in this population identified a prominent QTL on chromosome 5, which corresponded to Organophosphate-Sensitive Reaction (OSR) reported previously in other mapping populations. The OSR locus included a cluster of three genes potentially encoding nucleotide-binding leucine-rich repeat (NB-LRR, NLR) proteins, among which NLR-C was considered to be responsible for OPS in a dominant fashion. NLR-C was functional in NOG, whereas the other resistant parent, BTx623, had a null mutation caused by the deletion of promoter sequences. Our finding of OSR as a dominant trait is important not only in understanding the diversified role of NB-LRR proteins in cereals but also in securing sorghum breeding free from OPS.
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U2 - 10.1038/s41598-021-98908-7
DO - 10.1038/s41598-021-98908-7
M3 - Article
C2 - 34615901
AN - SCOPUS:85116415165
SN - 2045-2322
VL - 11
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 19828
ER -