Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells

Nobuaki Okumura; Masanori Ikeda; Shinya Satoh; Hiromichi Dansako; Masaya Sugiyama; Masashi Mizokami; Nobuyuki Kato

doi:10.1016/j.febslet.2015.03.022

Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells

Nobuaki Okumura, Masanori Ikeda, Shinya Satoh, Hiromichi Dansako, Masaya Sugiyama, Masashi Mizokami, Nobuyuki Kato

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Hepatitis B virus (HBV) replication is controlled by liver-enriched transcriptional factors, including forkhead box protein A (FOXA) members. Here, we found that FOXA members are directly and indirectly involved in HBV replication in human hepatic cells. HBV replication was elevated in HuH-7 treated with individual FOXA members-specific siRNA. Reciprocally, the downregulation of HBV replication was observed in FOXA-induced HuH-7. However, the mechanism of downregulation is different among FOXA members at the level of HBV RNA transcription, such as precore/pg RNA and 2.1 kb RNA. In addition, FOXA1 and FOXA2 suppressed nuclear hormone receptors, such as HNF4α, that are related to HBV replication.

Original language	English
Pages (from-to)	1112-1118
Number of pages	7
Journal	FEBS Letters
Volume	589
Issue number	10
DOIs	https://doi.org/10.1016/j.febslet.2015.03.022
Publication status	Published - Apr 28 2015

Keywords

FOXA1
FOXA2
FOXA3
HNF3
Hepatitis B virus
Hepatitis B virus replication

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2015.03.022

Cite this

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title = "Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells",

abstract = "Hepatitis B virus (HBV) replication is controlled by liver-enriched transcriptional factors, including forkhead box protein A (FOXA) members. Here, we found that FOXA members are directly and indirectly involved in HBV replication in human hepatic cells. HBV replication was elevated in HuH-7 treated with individual FOXA members-specific siRNA. Reciprocally, the downregulation of HBV replication was observed in FOXA-induced HuH-7. However, the mechanism of downregulation is different among FOXA members at the level of HBV RNA transcription, such as precore/pg RNA and 2.1 kb RNA. In addition, FOXA1 and FOXA2 suppressed nuclear hormone receptors, such as HNF4α, that are related to HBV replication.",

keywords = "FOXA1, FOXA2, FOXA3, HNF3, Hepatitis B virus, Hepatitis B virus replication",

author = "Nobuaki Okumura and Masanori Ikeda and Shinya Satoh and Hiromichi Dansako and Masaya Sugiyama and Masashi Mizokami and Nobuyuki Kato",

note = "Funding Information: We also thank Masayo Takemoto, Narumi Yamane, and Takashi Nakamura for their technical assistance. This work was supported by Grants-in-Aid for Research on Hepatitis from the Ministry of Health, Labor, and Welfare of Japan . Publisher Copyright: {\textcopyright} 2015 Federation of European Biochemical Societies.",

year = "2015",

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day = "28",

doi = "10.1016/j.febslet.2015.03.022",

language = "English",

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T1 - Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells

AU - Okumura, Nobuaki

AU - Ikeda, Masanori

AU - Satoh, Shinya

AU - Dansako, Hiromichi

AU - Sugiyama, Masaya

AU - Mizokami, Masashi

AU - Kato, Nobuyuki

N1 - Funding Information: We also thank Masayo Takemoto, Narumi Yamane, and Takashi Nakamura for their technical assistance. This work was supported by Grants-in-Aid for Research on Hepatitis from the Ministry of Health, Labor, and Welfare of Japan . Publisher Copyright: © 2015 Federation of European Biochemical Societies.

PY - 2015/4/28

Y1 - 2015/4/28

N2 - Hepatitis B virus (HBV) replication is controlled by liver-enriched transcriptional factors, including forkhead box protein A (FOXA) members. Here, we found that FOXA members are directly and indirectly involved in HBV replication in human hepatic cells. HBV replication was elevated in HuH-7 treated with individual FOXA members-specific siRNA. Reciprocally, the downregulation of HBV replication was observed in FOXA-induced HuH-7. However, the mechanism of downregulation is different among FOXA members at the level of HBV RNA transcription, such as precore/pg RNA and 2.1 kb RNA. In addition, FOXA1 and FOXA2 suppressed nuclear hormone receptors, such as HNF4α, that are related to HBV replication.

AB - Hepatitis B virus (HBV) replication is controlled by liver-enriched transcriptional factors, including forkhead box protein A (FOXA) members. Here, we found that FOXA members are directly and indirectly involved in HBV replication in human hepatic cells. HBV replication was elevated in HuH-7 treated with individual FOXA members-specific siRNA. Reciprocally, the downregulation of HBV replication was observed in FOXA-induced HuH-7. However, the mechanism of downregulation is different among FOXA members at the level of HBV RNA transcription, such as precore/pg RNA and 2.1 kb RNA. In addition, FOXA1 and FOXA2 suppressed nuclear hormone receptors, such as HNF4α, that are related to HBV replication.

KW - FOXA1

KW - FOXA2

KW - FOXA3

KW - HNF3

KW - Hepatitis B virus

KW - Hepatitis B virus replication

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ER -

Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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