TY - JOUR
T1 - Neofunctionalization of the secreted Tin2 effector in the fungal pathogen Ustilago maydis
AU - Tanaka, Shigeyuki
AU - Schweizer, Gabriel
AU - Rössel, Nicole
AU - Fukada, Fumi
AU - Thines, Marco
AU - Kahmann, Regine
N1 - Funding Information:
We are very grateful to S. Reissmann and E. Stukenbrock for critical reading and constructive comments on the manuscript, to the entire group in Marburg for helpful discussions, to K. Münch for help with plant infections and to D.K. Gupta and R. Sharma for bioinformatics support. Our work was supported by generous funds from the Max Planck Society and by the LOEWE initiative of the government of Hesse in the framework of the Centre for Translational Biodiversity Genomics (TBG).
Publisher Copyright:
© 2018, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Plant-pathogenic fungi hijack their hosts by secreting effector proteins. Effectors serve to suppress plant immune responses and modulate the host metabolism to benefit the pathogen. Smut fungi are biotrophic pathogens that also parasitize important cereals, including maize 1 . Symptom development is usually restricted to the plant inflorescences. Ustilago maydis is an exception in its ability to cause tumours in both inflorescences and leaves of maize, and in inducing anthocyanin biosynthesis through the secreted Tin2 effector 2,3 . How the unique lifestyle of U. maydis has evolved remains to be elucidated. Here we show that Tin2 in U. maydis has been neofunctionalized. We functionally compared Tin2 effectors of U. maydis and the related smut Sporisorium reilianum, which results in symptoms only in the inflorescences of maize and fails to induce anthocyanin. We show that Tin2 effectors from both fungi target distinct paralogues of a maize protein kinase, leading to stabilization and inhibition, respectively. An ancestral Tin2 effector functionally replaced the virulence function of S. reilianum Tin2 but failed to induce anthocyanin, and was unable to substitute for Tin2 in U. maydis. This shows that Tin2 in U. maydis has acquired a specialized function, probably connected to the distinct pathogenic lifestyle of this fungus.
AB - Plant-pathogenic fungi hijack their hosts by secreting effector proteins. Effectors serve to suppress plant immune responses and modulate the host metabolism to benefit the pathogen. Smut fungi are biotrophic pathogens that also parasitize important cereals, including maize 1 . Symptom development is usually restricted to the plant inflorescences. Ustilago maydis is an exception in its ability to cause tumours in both inflorescences and leaves of maize, and in inducing anthocyanin biosynthesis through the secreted Tin2 effector 2,3 . How the unique lifestyle of U. maydis has evolved remains to be elucidated. Here we show that Tin2 in U. maydis has been neofunctionalized. We functionally compared Tin2 effectors of U. maydis and the related smut Sporisorium reilianum, which results in symptoms only in the inflorescences of maize and fails to induce anthocyanin. We show that Tin2 effectors from both fungi target distinct paralogues of a maize protein kinase, leading to stabilization and inhibition, respectively. An ancestral Tin2 effector functionally replaced the virulence function of S. reilianum Tin2 but failed to induce anthocyanin, and was unable to substitute for Tin2 in U. maydis. This shows that Tin2 in U. maydis has acquired a specialized function, probably connected to the distinct pathogenic lifestyle of this fungus.
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U2 - 10.1038/s41564-018-0304-6
DO - 10.1038/s41564-018-0304-6
M3 - Letter
C2 - 30510169
AN - SCOPUS:85058048295
SN - 2058-5276
VL - 4
SP - 251
EP - 257
JO - Nature Microbiology
JF - Nature Microbiology
IS - 2
ER -