Neuroprotective and Therapeutic Effects of Tocovid and Twendee-X on Aβ Oligomer-Induced Damage in the SH-SY5Y Cell Line

Xiao Hu, Toru Yamashita, Haibo Yu, Zhihong Bian, Xinran Hu, Tian Feng, Koh Tadokoro, Ryuta Morihara, Koji Abe

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Background: Alzheimer's disease (AD) is the most frequent cause of dementia among the elderly. The accumulation of amyloid beta (Aβ) and its downstream pathological events such as oxidative stress play central roles in AD. Recent studies revealed that Aβ oligomer (AβO)-induced strong neurotoxicity in SH-SY5Y cells via the induction of oxidative stress. Objective: In the present study, we investigated the effect of two antioxidants, Tocovid and Twendee-X, on AβO-induced SH-SY5Y cell damage. Methods: AβOs (2.5 μM) were applied to induce cellular damage in the SH-SY5Y cell line. Cell viability following AβO toxicity, Tau protein phosphorylation, cell morphology, and intracellular reactive oxygen species were assayed with or without different concentrations of Tocovid or Twendee-X. Results: Tocovid (60 μg/mL) and Twendee-X (150 μg/mL) significantly recovered cell viability from AβO toxicity (∗∗p < 0.01, vs. control), attenuated Tau protein phosphorylation (∗∗p < 0.01, vs. AβOs), improved cell morphology (∗∗p < 0.01, vs. AβOs), and suppressed intracellular ROS (∗∗p < 0.01, vs. AβOs) in SH-SY5Y cells. Conclusion: These findings suggest the neuroprotective and therapeutic potential of Tocovid and Twendee-X for AD treatment.

Original languageEnglish
Pages (from-to)117-125
Number of pages9
JournalNeurodegenerative Diseases
Issue number5-6
Publication statusPublished - Jul 1 2022


  • Alzheimer's disease
  • Amyloid-β oligomer
  • SH-SY5Y cell
  • Tocovid
  • Twendee-X

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology


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