Neutrophil attractant/activation protein-1 (NAP-1) causes human basophil histamine release

Martha V. White; Teizo Yoshimura; William Hook; Michael A. Kaliner; Edward J. Leonard

doi:10.1016/0165-2478(89)90182-X

Neutrophil attractant/activation protein-1 (NAP-1) causes human basophil histamine release

Martha V. White, Teizo Yoshimura, William Hook, Michael A. Kaliner, Edward J. Leonard

Research output: Contribution to journal › Article › peer-review

91 Citations (Scopus)

Abstract

Basophils from five of six human donors released histamine in response to neutrophil attractant/activation protein-1 (NAP-1). Histamine release by this protein was concentration-dependent over the range of 3×10^-7 M to 4×10^-6 M. At 4×10^-6 M, the mean agonist-induced release was 16 ± 3% (SEM) of total basophil histamine. For the same basophil preparations, release by anti-IgE was 35 ± 6%. The chemotactic protein did not cause release of histamine from basophils at 0°C or in the presence of 10 mM EDTA. The time-course of histamine release was rapid; release was 43% of maximal after 30 s and maximal after 1 min of incubation. Thus, in addition to its previously characterized neutrophil chemotactic and activating properties, this protein activates human basophils.

Original language	English
Pages (from-to)	151-154
Number of pages	4
Journal	Immunology Letters
Volume	22
Issue number	2
DOIs	https://doi.org/10.1016/0165-2478(89)90182-X
Publication status	Published - Aug 1989
Externally published	Yes

Keywords

Basophils
Chemotaxis
Histamine release
Monocyte
Neutrophil
Neutrophil attractant/activation protein-1

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0165-2478(89)90182-X

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title = "Neutrophil attractant/activation protein-1 (NAP-1) causes human basophil histamine release",

abstract = "Basophils from five of six human donors released histamine in response to neutrophil attractant/activation protein-1 (NAP-1). Histamine release by this protein was concentration-dependent over the range of 3×10-7 M to 4×10-6 M. At 4×10-6 M, the mean agonist-induced release was 16 ± 3% (SEM) of total basophil histamine. For the same basophil preparations, release by anti-IgE was 35 ± 6%. The chemotactic protein did not cause release of histamine from basophils at 0°C or in the presence of 10 mM EDTA. The time-course of histamine release was rapid; release was 43% of maximal after 30 s and maximal after 1 min of incubation. Thus, in addition to its previously characterized neutrophil chemotactic and activating properties, this protein activates human basophils.",

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author = "White, {Martha V.} and Teizo Yoshimura and William Hook and Kaliner, {Michael A.} and Leonard, {Edward J.}",

year = "1989",

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doi = "10.1016/0165-2478(89)90182-X",

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AU - White, Martha V.

AU - Yoshimura, Teizo

AU - Hook, William

AU - Kaliner, Michael A.

AU - Leonard, Edward J.

PY - 1989/8

Y1 - 1989/8

N2 - Basophils from five of six human donors released histamine in response to neutrophil attractant/activation protein-1 (NAP-1). Histamine release by this protein was concentration-dependent over the range of 3×10-7 M to 4×10-6 M. At 4×10-6 M, the mean agonist-induced release was 16 ± 3% (SEM) of total basophil histamine. For the same basophil preparations, release by anti-IgE was 35 ± 6%. The chemotactic protein did not cause release of histamine from basophils at 0°C or in the presence of 10 mM EDTA. The time-course of histamine release was rapid; release was 43% of maximal after 30 s and maximal after 1 min of incubation. Thus, in addition to its previously characterized neutrophil chemotactic and activating properties, this protein activates human basophils.

AB - Basophils from five of six human donors released histamine in response to neutrophil attractant/activation protein-1 (NAP-1). Histamine release by this protein was concentration-dependent over the range of 3×10-7 M to 4×10-6 M. At 4×10-6 M, the mean agonist-induced release was 16 ± 3% (SEM) of total basophil histamine. For the same basophil preparations, release by anti-IgE was 35 ± 6%. The chemotactic protein did not cause release of histamine from basophils at 0°C or in the presence of 10 mM EDTA. The time-course of histamine release was rapid; release was 43% of maximal after 30 s and maximal after 1 min of incubation. Thus, in addition to its previously characterized neutrophil chemotactic and activating properties, this protein activates human basophils.

KW - Basophils

KW - Chemotaxis

KW - Histamine release

KW - Monocyte

KW - Neutrophil

KW - Neutrophil attractant/activation protein-1

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ER -

Neutrophil attractant/activation protein-1 (NAP-1) causes human basophil histamine release

Abstract

Keywords

ASJC Scopus subject areas

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