TY - JOUR
T1 - New efficient replication system with hepatitis C virus genome derived from a patient with acute hepatitis C
AU - Mori, Kyoko
AU - Abe, Ken ichi
AU - Dansako, Hiromichi
AU - Ariumi, Yasuo
AU - Ikeda, Masanori
AU - Kato, Nobuyuki
N1 - Funding Information:
We thank T. Nakamura for technical assistance. We also thank A. Takamizawa and M. Kohara for the anti-NS3, NS4A, NS5A, and NS5B antibodies. This work was supported by grants-in-aid for a third-term comprehensive 10-year strategy for cancer control, and for research on hepatitis from the Ministry of Health, Labor, and Welfare of Japan.
PY - 2008/6/20
Y1 - 2008/6/20
N2 - We report for the first time a new RNA replication system with a hepatitis C virus (HCV) strain (AH1) derived from a patient with acute hepatitis C. Using an HCV replicon RNA library constructed with the AH1 strain (genotype 1b), we first established a cloned cell line, sAH1, harboring the HCV replicon. Cured cells obtained with interferon treatment of sAH1 cells were used for transfection with genome-length HCV RNA possessing four mutations found in sAH1 replicon. Consequently, one cloned cell line, AH1, supporting efficient replication of genome-length HCV RNA was obtained. By the comparison of AH1 cells with the O cells supporting genome-length HCV RNA (HCV-O strain) replication, we found different anti-HCV profiles of interferon-γ and cyclosporine A between AH1 and O cells. Reporter assay analysis suggests that the diverse effects of interferon-γ are due to the difference in HCV strains, but not the cellular environment.
AB - We report for the first time a new RNA replication system with a hepatitis C virus (HCV) strain (AH1) derived from a patient with acute hepatitis C. Using an HCV replicon RNA library constructed with the AH1 strain (genotype 1b), we first established a cloned cell line, sAH1, harboring the HCV replicon. Cured cells obtained with interferon treatment of sAH1 cells were used for transfection with genome-length HCV RNA possessing four mutations found in sAH1 replicon. Consequently, one cloned cell line, AH1, supporting efficient replication of genome-length HCV RNA was obtained. By the comparison of AH1 cells with the O cells supporting genome-length HCV RNA (HCV-O strain) replication, we found different anti-HCV profiles of interferon-γ and cyclosporine A between AH1 and O cells. Reporter assay analysis suggests that the diverse effects of interferon-γ are due to the difference in HCV strains, but not the cellular environment.
KW - Acute hepatitis C
KW - Anti-HCV reagents
KW - Genome-length HCV RNA
KW - HCV replication system
KW - Hepatitis C virus
KW - Interferon-γ
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U2 - 10.1016/j.bbrc.2008.04.005
DO - 10.1016/j.bbrc.2008.04.005
M3 - Article
C2 - 18406345
AN - SCOPUS:46349106552
SN - 0006-291X
VL - 371
SP - 104
EP - 109
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -
