@article{554cba8df52d4e8e8cd48cb2671dff49,
title = "New insights into the dimerization of small GTPase Rac/ROP guanine nucleotide exchange factors in rice",
abstract = "Molecular links between receptor-kinases and Rac/ROP family small GTPases mediated by activator guanine nucleotide exchange factors (GEFs) govern diverse biological processes. However, it is unclear how the Rac/ROP GTPases orchestrate such a wide variety of activities. Here, we show that rice OsRacGEF1 forms homodimers, and heterodimers with OsRacGEF2, at the plasma membrane (PM) and the endoplasmic reticulum (ER). OsRacGEF2 does not bind directly to the receptor-like kinase (RLK) OsCERK1, but forms a complex with OsCERK1 through OsRacGEF1 at the ER. This complex is transported from ER to the PM and there associates with OsRac1, resulting in the formation of a stable immune complex. Such RLK-GEF heterodimer complexes may explain the diversity of Rac/ROP family GTPase signalings.",
keywords = "PRONE GEF, Rac/ROP GTPase, dimerization, membrane trafficking",
author = "Akira Akamatsu and Kazumi Uno and Midori Kato and Wong, {Hann Ling} and Ko Shimamoto and Yoji Kawano",
note = "Funding Information: A.A. was supported by a fellowship from the Japan Society for the Promotion of Science (JSPS). This research was supported by MAFF Genomics for Agricultural Innovation Grant Number PMI0007 to K.S., by JSPS KAKENHI Grant Numbers 19108005 to K.S. and 2377044, 24113515, 26113712 and 26450055 to Y.K., by NAIST Foundation to Y. K., and by the Takeda Science Foundation to Y.K. We thank Dr. Masaru Fujimoto for providing GFP-L12, GFP-ATPase delta-prime subunit and GFP-PTS1, and also Drs. Akihiko Nakano, Takashi Ueda and Masaru Fujimoto for the organelle markers GFP-Erd2, GFP-SYP61 and GFP-ARA6. We appreciate Dr. Minoru Nagano for insightful discussion and Ms. Tomoko Aoi for excellent technical assistance. We also thank the members of the Laboratory of Plant Molecular Genetics for invaluable comments and discussions. Funding Information: A.A. was supported by a fellowship from the Japan Society for the Promotion of Science (JSPS). This research was supported by MAFF Genomics for Agricultural Innovation Grant Number PMI0007 to K.S., by JSPS KAKENHI Grant Numbers 19108005 to K.S. and 2377044, 24113515, 26113712 and 26450055 to Y.K., by NAIST Foundation to Y. K., and by the Takeda Science Foundation to Y.K. Publisher Copyright: {\textcopyright} 2015, {\textcopyright} 2015 Taylor & Francis Group, LLC. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2015",
month = jul,
day = "3",
doi = "10.1080/15592324.2015.1044702",
language = "English",
volume = "10",
journal = "Plant Signaling and Behavior",
issn = "1559-2316",
publisher = "Landes Bioscience",
number = "7",
}