TY - JOUR
T1 - Nicotinamide mononucleotide and related metabolites induce disease resistance against fungal phytopathogens in Arabidopsis and barley
AU - Miwa, Akihiro
AU - Sawada, Yuji
AU - Tamaoki, Daisuke
AU - Hirai, Masami Yokota
AU - Kimura, Makoto
AU - Sato, Kazuhiro
AU - Nishiuchi, Takumi
N1 - Funding Information:
This research was supported by grants from the Ministry of Agriculture, Forestry and Fisheries of Japan TRS1005, Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry (28007A), and the Joint Research Program implemented at the Institute of Plant Science and Resources, Okayama University in Japan. Barley seed samples were provided by the National Bio-Resource Project of the Ministry of Education, Culture, Sports, Science and Technology, Japan. This work was also supported in part by Research for Promoting Technological Seeds (no. 07-070) and by the Adaptable and Seamless Technology Transfer Program through Target-driven R&D (AS232Z02753E and AS242Z03390N) from the Japan Science and Technology Agency (JST). This work was partly supported by KAKENHI (no. 23580060, 26450053 and 15H05780) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Muneo Sato, Yutaka Yamada, and Akane Sakata (RIKEN) for technical assistance in metabolomics. We are deeply grateful to Drs. Shigeru Shigeoka and Masahiro Tamoi (Kinki University) and Dr. Maki Kawai-Yamada for their helpful comments.
Publisher Copyright:
© 2017 The Author(s).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Nicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD), is known to act as a functional molecule in animals, whereas its function in plants is largely unknown. In this study, we found that NMN accumulated in barley cultivars resistant to phytopathogenic fungal Fusarium species. Although NMN does not possess antifungal activity, pretreatment with NMN and related metabolites enhanced disease resistance to Fusarium graminearum in Arabidopsis leaves and flowers and in barley spikes. The NMN-induced Fusarium resistance was accompanied by activation of the salicylic acid-mediated signalling pathway and repression of the jasmonic acid/ethylene-dependent signalling pathways in Arabidopsis. Since NMN-induced disease resistance was also observed in the SA-deficient sid2 mutant, an SA-independent signalling pathway also regulated the enhanced resistance induced by NMN. Compared with NMN, NAD and NADP, nicotinamide pretreatment had minor effects on resistance to F. graminearum. Constitutive expression of the NMNAT gene, which encodes a rate-limiting enzyme for NAD biosynthesis, resulted in enhanced disease resistance in Arabidopsis. Thus, modifying the content of NAD-related metabolites can be used to optimize the defence signalling pathways activated in response to F. graminearum and facilitates the control of disease injury and mycotoxin accumulation in plants.
AB - Nicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD), is known to act as a functional molecule in animals, whereas its function in plants is largely unknown. In this study, we found that NMN accumulated in barley cultivars resistant to phytopathogenic fungal Fusarium species. Although NMN does not possess antifungal activity, pretreatment with NMN and related metabolites enhanced disease resistance to Fusarium graminearum in Arabidopsis leaves and flowers and in barley spikes. The NMN-induced Fusarium resistance was accompanied by activation of the salicylic acid-mediated signalling pathway and repression of the jasmonic acid/ethylene-dependent signalling pathways in Arabidopsis. Since NMN-induced disease resistance was also observed in the SA-deficient sid2 mutant, an SA-independent signalling pathway also regulated the enhanced resistance induced by NMN. Compared with NMN, NAD and NADP, nicotinamide pretreatment had minor effects on resistance to F. graminearum. Constitutive expression of the NMNAT gene, which encodes a rate-limiting enzyme for NAD biosynthesis, resulted in enhanced disease resistance in Arabidopsis. Thus, modifying the content of NAD-related metabolites can be used to optimize the defence signalling pathways activated in response to F. graminearum and facilitates the control of disease injury and mycotoxin accumulation in plants.
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U2 - 10.1038/s41598-017-06048-8
DO - 10.1038/s41598-017-06048-8
M3 - Article
C2 - 28743869
AN - SCOPUS:85026230433
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 6389
ER -