Nicotine-induced CCN2: From smoking to periodontal fibrosis

H. Takeuchi, S. Kubota, E. Murakashi, Y. Zhou, K. Endo, P. S. Ng, M. Takigawa, Y. Numabe

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


Since fibrosis is observed in smokers gingiva, it was hypothesized that fibrosis was caused by nicotine in the periodontium. Therefore, in this study, we investigated the effects of nicotine on the induction of a profibrotic molecule, connective tissue growth factor (CCN2/CTGF), in human gingival fibroblasts (HGFs) and periodontal ligament (PDL) cells. With 1 μg/mL nicotine, vacuolization and attenuated proliferation were observed. Interestingly, 1 μg/mL nicotine increased the production of CCN2/CTGF protein in both cells without increasing mRNA expression. Furthermore, type I collagen mRNA and protein were also increased and were significantly blocked by a CCN2/CTGF neutralizing antibody. This is the first report to describe a relationship between nicotine and CCN2/CTGF in periodontal tissue cells. Analysis of our data also indicated that nicotine was cytotoxic, while it increased CCN2/CTGF and, eventually, type I collagen production. These findings suggest that periodontal fibrosis can be promoted by nicotine from smoking via effects on CCN2/CTGF.

Original languageEnglish
Pages (from-to)34-39
Number of pages6
JournalJournal of dental research
Issue number1
Publication statusPublished - Jan 2010


  • CTGF
  • Fibrosis
  • Gingival.
  • Nicotine
  • Smoking

ASJC Scopus subject areas

  • General Dentistry


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