Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro

Hideaki Abe; Wataru Ishikawa; Takahiro Kushima; Tomoka Nishimura; Chiemi Mori; Atsushi Onuki; Takehito Suzuki; Yasuo Ishii; Norio Kansaku; Yoko Miyazaki; Kazuaki Tanaka; Hidetoshi Morita; Tatsuya Takizawa

doi:10.1271/bbb.120923

Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro

Hideaki Abe, Wataru Ishikawa, Takahiro Kushima, Tomoka Nishimura, Chiemi Mori, Atsushi Onuki, Takehito Suzuki, Yasuo Ishii, Norio Kansaku, Yoko Miyazaki, Kazuaki Tanaka, Hidetoshi Morita, Tatsuya Takizawa

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

We investigated the role of nitric oxide (NO) in vascular endothelial growth factor (VEGF) expression in the rat placenta. A nitric oxide synthase (NOS) inhibitor, NG-nitro-L-arginine-methyl ester (L-NAME), was constantly infused into pregnant rats 6-24 h before sacrifice on gestational day (GD) 15.5. NO production declined to about 15% of the control level as monitored by NO trapping and electron paramagnetic resonance spectroscopy. VEGF mRNA expression was temporally decreased by L-NAME, but recovered to normal levels after 24 h of treatment, whereas hypoxia inducible factor (HIF)-1α and induced NOS (iNOS) expression increased. VEGF expression decreased significantly in placental explants after 6 h of co-treatment with LNAME and lipopolysaccharide, an iNOS inducer. Our data indicate that NO induce VEGF expression in vivo and in vitro in the rat placenta, suggesting that peaked NO production was maintained by a reciprocal relationship between NO and VEGF via HIF-1α.

Original language	English
Pages (from-to)	971-976
Number of pages	6
Journal	Bioscience, Biotechnology and Biochemistry
Volume	77
Issue number	5
DOIs	https://doi.org/10.1271/bbb.120923
Publication status	Published - 2013
Externally published	Yes

Keywords

Angiogenesis
Electron paramagnetic resonance (EPR) spectroscopy
Fetal rat placenta
Nitric oxide (NO)

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.120923

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Abe, H., Ishikawa, W., Kushima, T., Nishimura, T., Mori, C., Onuki, A., Suzuki, T., Ishii, Y., Kansaku, N., Miyazaki, Y., Tanaka, K., Morita, H., & Takizawa, T. (2013). Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro. Bioscience, Biotechnology and Biochemistry, 77(5), 971-976. https://doi.org/10.1271/bbb.120923

Abe, H, Ishikawa, W, Kushima, T, Nishimura, T, Mori, C, Onuki, A, Suzuki, T, Ishii, Y, Kansaku, N, Miyazaki, Y, Tanaka, K, Morita, H & Takizawa, T 2013, 'Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro', Bioscience, Biotechnology and Biochemistry, vol. 77, no. 5, pp. 971-976. https://doi.org/10.1271/bbb.120923

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title = "Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro",

abstract = "We investigated the role of nitric oxide (NO) in vascular endothelial growth factor (VEGF) expression in the rat placenta. A nitric oxide synthase (NOS) inhibitor, NG-nitro-L-arginine-methyl ester (L-NAME), was constantly infused into pregnant rats 6-24 h before sacrifice on gestational day (GD) 15.5. NO production declined to about 15% of the control level as monitored by NO trapping and electron paramagnetic resonance spectroscopy. VEGF mRNA expression was temporally decreased by L-NAME, but recovered to normal levels after 24 h of treatment, whereas hypoxia inducible factor (HIF)-1α and induced NOS (iNOS) expression increased. VEGF expression decreased significantly in placental explants after 6 h of co-treatment with LNAME and lipopolysaccharide, an iNOS inducer. Our data indicate that NO induce VEGF expression in vivo and in vitro in the rat placenta, suggesting that peaked NO production was maintained by a reciprocal relationship between NO and VEGF via HIF-1α.",

keywords = "Angiogenesis, Electron paramagnetic resonance (EPR) spectroscopy, Fetal rat placenta, Nitric oxide (NO)",

author = "Hideaki Abe and Wataru Ishikawa and Takahiro Kushima and Tomoka Nishimura and Chiemi Mori and Atsushi Onuki and Takehito Suzuki and Yasuo Ishii and Norio Kansaku and Yoko Miyazaki and Kazuaki Tanaka and Hidetoshi Morita and Tatsuya Takizawa",

note = "Funding Information: We thank Dr. Hiroshi Yoshikawa and Dr. Yoshinori Ikeda for conducting EPR spectroscopy analyses. This work was supported in part by a Grant-in-Aid for Scientific Research (no. 23580413) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and by a Grant-in-Aid for Matching Fund Subsidies for Private Universities from the Promotion and Mutual Aid Corporation for Private Schools of Japan.",

year = "2013",

doi = "10.1271/bbb.120923",

language = "English",

volume = "77",

pages = "971--976",

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publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "5",

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T1 - Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro

AU - Abe, Hideaki

AU - Ishikawa, Wataru

AU - Kushima, Takahiro

AU - Nishimura, Tomoka

AU - Mori, Chiemi

AU - Onuki, Atsushi

AU - Suzuki, Takehito

AU - Ishii, Yasuo

AU - Kansaku, Norio

AU - Miyazaki, Yoko

AU - Tanaka, Kazuaki

AU - Morita, Hidetoshi

AU - Takizawa, Tatsuya

N1 - Funding Information: We thank Dr. Hiroshi Yoshikawa and Dr. Yoshinori Ikeda for conducting EPR spectroscopy analyses. This work was supported in part by a Grant-in-Aid for Scientific Research (no. 23580413) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and by a Grant-in-Aid for Matching Fund Subsidies for Private Universities from the Promotion and Mutual Aid Corporation for Private Schools of Japan.

PY - 2013

Y1 - 2013

N2 - We investigated the role of nitric oxide (NO) in vascular endothelial growth factor (VEGF) expression in the rat placenta. A nitric oxide synthase (NOS) inhibitor, NG-nitro-L-arginine-methyl ester (L-NAME), was constantly infused into pregnant rats 6-24 h before sacrifice on gestational day (GD) 15.5. NO production declined to about 15% of the control level as monitored by NO trapping and electron paramagnetic resonance spectroscopy. VEGF mRNA expression was temporally decreased by L-NAME, but recovered to normal levels after 24 h of treatment, whereas hypoxia inducible factor (HIF)-1α and induced NOS (iNOS) expression increased. VEGF expression decreased significantly in placental explants after 6 h of co-treatment with LNAME and lipopolysaccharide, an iNOS inducer. Our data indicate that NO induce VEGF expression in vivo and in vitro in the rat placenta, suggesting that peaked NO production was maintained by a reciprocal relationship between NO and VEGF via HIF-1α.

AB - We investigated the role of nitric oxide (NO) in vascular endothelial growth factor (VEGF) expression in the rat placenta. A nitric oxide synthase (NOS) inhibitor, NG-nitro-L-arginine-methyl ester (L-NAME), was constantly infused into pregnant rats 6-24 h before sacrifice on gestational day (GD) 15.5. NO production declined to about 15% of the control level as monitored by NO trapping and electron paramagnetic resonance spectroscopy. VEGF mRNA expression was temporally decreased by L-NAME, but recovered to normal levels after 24 h of treatment, whereas hypoxia inducible factor (HIF)-1α and induced NOS (iNOS) expression increased. VEGF expression decreased significantly in placental explants after 6 h of co-treatment with LNAME and lipopolysaccharide, an iNOS inducer. Our data indicate that NO induce VEGF expression in vivo and in vitro in the rat placenta, suggesting that peaked NO production was maintained by a reciprocal relationship between NO and VEGF via HIF-1α.

KW - Angiogenesis

KW - Electron paramagnetic resonance (EPR) spectroscopy

KW - Fetal rat placenta

KW - Nitric oxide (NO)

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Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro

Abstract

Keywords

ASJC Scopus subject areas

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