Nitric oxide scavenging rates of solubilized resveratrol and flavonoids

Yoshimi Sueishi, Masashi Hori

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Relative nitric oxide scavenging rates of water-insoluble lipophilic antioxidants, such as resveratrol and flavonoids were determined using a β-cyclodextrin analog, DM-β-CD (heptakis(2,6-di-O-methyl)-β- cyclodextrin) as a solubilizer. Antioxidant-mediated decrease of NO concentration was measured with the electron spin resonance-based PTIO (2-phenyl-4,4,5,5-methylimidazoline-1-oxyl 3-oxide) method, from which scavenging rates were calculated. Because both the antioxidant and PTIO form inclusion complex with DM-β-CD, a kinetic treatment was necessary to calculate the scavenging rates. Resveratrol showed the highest NO scavenging rate among the tested antioxidants. The magnitudes of scavenging rates were in the order of: resveratrol > catechin ≈ myricetin > epicatechin > epigallocatechin gallate > kaempferol. This order is not in agreement with the oxygen radical scavenging rates: i.e., myricetin > epigallocatechin gallate > catechin ≈ epicatechin > resveratrol > kaempferol. The tested antioxidants showed lower scavenging rates than hydrophilic antioxidants such as uric acid and caffeic acids. Previous redox potential measurement of antioxidants revealed that NO scavenging occurs through non-free radical mechanism, indicating that the scavenging capacity is dependent on the nature of the scavenging reaction.

Original languageEnglish
Pages (from-to)25-29
Number of pages5
JournalNitric Oxide - Biology and Chemistry
Publication statusPublished - Feb 28 2013


  • 2,6-di-O-methylated β-cyclodextrin
  • Lipophilic antioxidant
  • NO-scavenging rate
  • Nitric oxide
  • PTIO

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Clinical Biochemistry
  • Cancer Research


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