Nitric oxide system is involved in glomerular hyperfiltration in Japanese normo- and micro-albuminuric patients with type 2 diabetes

Keita Hiragushi, Hikaru Sugimoto, Kenichi Shikata, Tetsuji Yamashita, Nobuyuki Miyatake, Yasushi Shikata, Jun Wada, Isao Kumagai, Masaki Fukushima, Hirofumi Makino

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


Glomerular hyperfiltration plays a pathogenic role in the early stages of diabetic nephropathy. Experimental studies in laboratory animals suggest that nitric oxide (NO) might be involved in the pathogenesis of glomerular hyperfiltration. We performed a cross-sectional study to determine the relationship between diabetic glomerular hyperfiltration and the NO system. Normoalbuminuric (n = 41), microalbuminuric (n = 25), and macroalbuminuric (n = 16) patients with type 2 diabetes were recruited in this study and compared with age-matched 84 non-diabetic control subjects. Creatinine clearance and urinary NO2-/NO3- excretion (urinary NOx) were measured, and the expression of endothelial cell nitric oxide synthase (ecNOS) was evaluated in human renal tissues. Glomerular hyperfiltration was present in 19 (37.5%) and nine (36.6%) of normoalbuminuric and microalbuminuric type 2 diabetic patients, respectively. The urinary NOx was significantly higher in normoalbuminuric patients compared with normal subjects. Creatinine clearance correlated significantly with urinary NOx in normoalbuminuric and microalbuminuric patients. Immunohistochemical staining intensities for ecNOS were significantly increased in glomerular endothelial cells of microalbuminuric type 2 diabetic patients as compared with the control subjects. These results suggest that NO may contribute to the pathogenesis of glomerular hyperfiltration in Japanese type 2 diabetic patients.

Original languageEnglish
Pages (from-to)149-159
Number of pages11
JournalDiabetes Research and Clinical Practice
Issue number3
Publication statusPublished - 2001


  • Diabetic nephropathy
  • Endothelial cell nitric oxide synthase
  • Glomerular hyperfiltration
  • Nitric oxide
  • Type 2 diabetes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology


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